| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,357,755 | 0 | A | T | 52.2% | 8.7 / 27.3 | 23 | P530P (CCA→CCT) | yhcD | fimbrial biogenesis outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base A (6/5); new base T (0/12); total (6/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357681‑3357836 | tGGCACAATAAACTTAGTGTTTAAAAAAAAAAGGAAGAGGATGGTGATCTGTTTTACGTCAACATCAGTTTTCTTTTTGGCAGAAAAAac < 1:662356/90‑1 (MQ=255) ccagaaTCACCTTAGTGCTTCAAAAAATAAGGAAGACGATGGTGATTTGTTCTCCGTAAACATCAGTATCCCATTTGGAAGATAAAacac < 1:815135/89‑1 (MQ=255) gcaTCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTTTTCTACGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGc < 1:802406/90‑1 (MQ=255) ccaTCAACTTAGTCCTTCAAAAAAAAATGAAGACGATGGTGATCTGTTCTACGTCAACATAAGTATTCCTTTTGGCAGATAAAACACCGc < 1:390197/89‑1 (MQ=255) aTCAACTTAGTGCTTCAGAAAATAATGAAGACGAGGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCca < 2:312505/90‑1 (MQ=255) aTCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCca < 2:819361/90‑1 (MQ=255) aTCAACTTAGTGCTTCAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCca < 1:276233/90‑1 (MQ=255) tCAACTTAGTGCTTCAAAAAAAAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCcac < 1:28544/90‑1 (MQ=255) aaCTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCacac < 2:142708/90‑1 (MQ=255) aaCTTAGTGCTTCAAAAAAAAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATCAAACACCGCCacac < 1:397054/90‑1 (MQ=255) aCTTAGTGCTTAAAAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCTTTTGGCAGATAAAACACCGCCACACt < 1:78103/90‑1 (MQ=255) tCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGc > 2:488000/1‑90 (MQ=255) gATCTGTTCTCCGTCAACATCAGTTTTCCTTTTGGAAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTAt < 1:489967/90‑1 (MQ=255) gTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCca > 1:25205/1‑47 (MQ=255) gTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCca < 2:25205/47‑1 (MQ=255) tttcGTCAAAAACATTTTTCCTTTTGGCAAAAAAAACCCCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTgg < 2:846237/87‑1 (MQ=255) cTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTgg > 2:920418/1‑90 (MQ=255) aCGTCAACATCAGTATTCCATTTGGCAGATCAAac > 1:843465/1‑35 (MQ=255) aCGTCAACATCAGTATTCCATTTGGCAGATCAAac < 2:843465/35‑1 (MQ=255) aCGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGgtg > 2:274446/1‑90 (MQ=255) aaCATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACg < 2:15727/58‑1 (MQ=255) aaCATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACg > 1:15727/1‑58 (MQ=255) aGTATTCCTTTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAgtc < 1:274446/90‑1 (MQ=255) | TGGCAGCATCAACTTAGTGCTTCAGAAAATAATGAAGACGATGGTGATCTGTTCTACGTCAACATCAGTATTCCATTTGGCAGATCAAACACCGCCACACTGTATACTCGCCATGACGATCATAAAACCCACTATGGTACTGGTGTCATGGGAGTC > NZ_CP009273/3357681‑3357836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |