| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,703,332 | 0 | A | C | 55.6% | 12.5 / 22.2 | 18 | G168G (GGT→GGG) | eptB | kdo(2)‑lipid A phosphoethanolamine 7''‑transferase |
| Reads supporting (aligned to +/- strand): ref base A (8/0); new base C (0/10); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTAT > NZ_CP009273/3703243‑3703417 | ccGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ataata > 2:712391/1‑90 (MQ=255)ccGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ataata > 2:312964/1‑90 (MQ=255)ccGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ataata > 2:296093/1‑90 (MQ=255)ccGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ataata > 2:162808/1‑90 (MQ=255) aTAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGc > 1:490802/1‑90 (MQ=255) cGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTac > 2:81760/1‑90 (MQ=255) ggCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTacg > 2:374910/1‑90 (MQ=255) cGCCGGCCCCCCCCCCCCTTTTCTTCTGGTGGATACCCAGAAAACGAATGGGTCCCAAAACC‑ATAATCCCCGCCAGTACGACGACCGCCa < 2:753533/90‑1 (MQ=255) ggTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCt > 1:838960/1‑90 (MQ=255) ggTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCt > 1:83045/1‑90 (MQ=255) cccccCTCCCCTTTCTTCTGGTGGATACCCAAAAAACAAATCGGCCCCCAAACA‑AAAACCCCCGCCAGTACGACGACCGCCAGGCTGCGa < 2:515177/89‑1 (MQ=255) ccctcccctcTTTCTTCTCCGGAATACCAAAAAAACAAAGGGGCCCCAAACCC‑AAAACCCCCGCCAGTACGACGACCGCCAGGCTGCGaa < 1:460434/90‑1 (MQ=255) cccTCCCCCCTTTCTTCTGCGGGATACCCAGCAAACAAATCGGTCCCCAACCC‑ATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGaa < 2:33836/90‑1 (MQ=255) tctcCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATAc > 2:590356/1‑90 (MQ=255) tcCCCTTTCTTCTGCTGAATCGCCACAAAACGAGTCGGTCCCAAAACC‑ATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGc < 2:706346/90‑1 (MQ=255) ccctcTTTCTTCTGCGGGATACCAACAAAACGAATGGGTCCCAAAACA‑ATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGc < 2:544151/86‑1 (MQ=255) cccccttccttcTGCTGAATACCCAAAAAACGAA‑GGGGCCCCAAACCAAAAACCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGc < 1:590356/86‑1 (MQ=255) ttttCTTCTGCGGAAAACCCACAAAAGGAATGGGGCCCAAAACA‑ATAACCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGcc < 1:263187/89‑1 (MQ=255) ttctGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTggg > 1:787023/1‑90 (MQ=255) ccAGAAAACGAATGGGTCCCAAAACA‑ATAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTc < 2:83045/90‑1 (MQ=255) caaaaCA‑AAAATCCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACa < 2:739768/88‑1 (MQ=255) c‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGtt > 1:400272/1‑90 (MQ=255) taataCCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTAt > 1:482336/1‑90 (MQ=255) | CCGCCATAACTCGGCAAATCAACGCCGGTCGCCCTCTCCACTTTCTTCTGCTGGATATCCAGCAAACGAATCGGTGCCCAAACC‑ATAATACCCGCCAGTACGACGACCGCCAGGCTGCGAATACGCTGCCCTGGGGTTCGCAGTTGTCGGAGCAAGGTGTAGCGACAGCGGTTAT > NZ_CP009273/3703243‑3703417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |