Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→G	D27E (GAT→GAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	G	100.0%	111.5 / NA	32	D27E (GAT→GAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/17);  total (15/17)

TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982                                                                                    |                                                                                tcAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc                                                                           <  2:813497/90‑1 (MQ=255)        tCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTa                                                                    >  2:871042/1‑90 (MQ=255)         cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaa                                                                   >  2:82225/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                  >  1:769660/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaaa                                                                  >  1:800945/1‑90 (MQ=255)          aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCCAGCTCGCCTGGTTTaaa                                                                  >  2:918573/1‑90 (MQ=255)              tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCa                                                              <  2:738742/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                             <  2:238363/90‑1 (MQ=255)               gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                             <  2:115216/90‑1 (MQ=255)                aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            <  2:127819/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        <  2:536864/90‑1 (MQ=255)                    cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        <  1:720424/90‑1 (MQ=255)                     ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCtt                                                       >  1:607189/1‑90 (MQ=255)                      gcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTta                                                      <  1:142661/90‑1 (MQ=255)                                tAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGGTTAAACGCAACACCTTAAATAAACCCg                                            >  1:181631/1‑90 (MQ=255)                                 aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  2:607189/90‑1 (MQ=255)                                  gATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                          >  1:3380/1‑90 (MQ=255)                                      gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                      <  2:3380/90‑1 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                             >  2:278851/1‑64 (MQ=255)                                         ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCaa                                                             <  1:278851/64‑1 (MQ=255)                                             cGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                               <  2:181631/90‑1 (MQ=255)                                                         cGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   >  1:656739/1‑90 (MQ=255)                                                         cGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   <  1:622973/90‑1 (MQ=255)                                                         cGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                   <  1:82225/90‑1 (MQ=255)                                                             aTGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            <  2:419838/45‑1 (MQ=255)                                                             aTGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            >  1:419838/1‑45 (MQ=255)                                                                  gTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        <  2:594363/44‑1 (MQ=255)                                                                  gTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        >  1:594363/1‑44 (MQ=255)                                                                    ggAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc        <  2:840639/90‑1 (MQ=255)                                                                         ctgcctgcCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg   >  2:538769/1‑90 (MQ=255)                                                                          tgcctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  >  2:71827/1‑90 (MQ=255)                                                                          tgcctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  >  1:192259/1‑90 (MQ=255)                                                                                    |                                                                                TCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49820‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑