Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,279	T→C	F153L (TTT→CTT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,279	0	T	C	100.0%	104.4 / NA	27	F153L (TTT→CTT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

CCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC  >  NZ_CP009273/50197‑50364                                                                                   |                                                                                      cccGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGAt                                                                                <  1:382911/90‑1 (MQ=255)                    gACTGGGAATCGGTATTCAGCGAATTTCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAAttt                                                             <  1:318984/90‑1 (MQ=255)                    gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAAttt                                                             <  1:160475/90‑1 (MQ=255)                    gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAAttt                                                             <  1:828908/90‑1 (MQ=255)                    gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAAttt                                                             <  1:386382/90‑1 (MQ=255)                      cTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTg                                                           >  1:1665/1‑90 (MQ=255)                          gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTtata                                                       >  2:497218/1‑90 (MQ=255)                          gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGtata                                                       <  1:534173/90‑1 (MQ=255)                          gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGtata                                                       <  1:792789/90‑1 (MQ=255)                          gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGtata                                                       <  1:20944/90‑1 (MQ=255)                          gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGGAATTTTGtata                                                       >  2:64732/1‑90 (MQ=255)                               ggTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAAtt                                                  >  1:162484/1‑90 (MQ=255)                                          aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTgg                                                                           <  1:437808/54‑1 (MQ=255)                                          aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTgg                                                                           >  2:437808/1‑54 (MQ=255)                                            ttCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGcc                                     >  2:393749/1‑90 (MQ=255)                                                        gATGCGCAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGcc                         <  1:64732/90‑1 (MQ=255)                                                             gcAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTATGGCTAACGCCCGATGGGGCggcgggcg                    >  2:705952/1‑90 (MQ=255)                                                             gcAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTcg                    <  2:328492/90‑1 (MQ=255)                                                             gcAGAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGGAATTTTTTATAGAATTTTCGGCTAGCGCCCGGTGGGGCGCCGGGcg                    >  1:13350/1‑90 (MQ=255)                                                                gAACTCTCACAGCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCCGGCGCGt                 >  2:2579/1‑90 (MQ=255)                                                                           gCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc      <  1:455112/90‑1 (MQ=255)                                                                           gCTATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc      <  2:827954/90‑1 (MQ=255)                                                                             tATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCtt    <  2:1665/90‑1 (MQ=255)                                                                             tATTGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGGCGCGTCTTATCCCGGCtt    >  2:2503/1‑90 (MQ=39)                                                                               ttGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  <  2:314122/90‑1 (MQ=255)                                                                               ttGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  >  2:310988/1‑90 (MQ=255)                                                                               ttGCCTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc  <  2:810289/90‑1 (MQ=255)                                                                                   |                                                                                      CCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCC  >  NZ_CP009273/50197‑50364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑