| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,613,723 | G→C | G116A (GGC→GCC) | marR → | multiple antibiotic resistance transcriptional regulator MarR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,613,723 | 0 | G | C | 90.0% | 66.5 / 1.0 | 20 | G116A (GGC→GCC) | marR | multiple antibiotic resistance transcriptional regulator MarR |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (7/11); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTA > NZ_CP009273/1613636‑1613810 | tGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGccc > 1:637231/1‑90 (MQ=255) gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCa < 2:616683/90‑1 (MQ=255) aCCCGAATGACAAGCGCGGCCTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGAcc < 2:890646/90‑1 (MQ=255) cccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCt < 2:822552/90‑1 (MQ=255) tGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCa < 2:524095/90‑1 (MQ=255) gACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCaa < 1:321071/90‑1 (MQ=255) ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg > 1:784510/1‑90 (MQ=255) ttACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg > 1:813607/1‑90 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCa < 1:335433/62‑1 (MQ=255) accaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCa > 2:335433/1‑62 (MQ=255) ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCa < 2:165079/90‑1 (MQ=255) ggcgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCa < 1:510056/90‑1 (MQ=255) cgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac < 2:300237/90‑1 (MQ=255) cgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac < 2:196163/90‑1 (MQ=255) cgcggcAATATGTGAACAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac < 2:893414/90‑1 (MQ=255) aaCAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGc > 2:564459/1‑90 (MQ=255) cAATGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCtt < 2:224797/90‑1 (MQ=255) aaTGCCATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTa > 2:783646/1‑90 (MQ=255) ccATCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGaa > 2:755598/1‑90 (MQ=255) aTCAATTAGTTGCCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAg > 1:76011/1‑90 (MQ=255) tGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTa > 1:33467/1‑90 (MQ=255) | TGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTA > NZ_CP009273/1613636‑1613810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |