Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273585,3860AG61.5% 1.8 / 11.1 13L342P (CTC→CCC) nfrAbacteriophage adsorption protein NfrA
Reads supporting (aligned to +/- strand):  ref base A (0/5);  new base G (8/0);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 7.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTG  >  NZ_CP009273/585319‑585472
                                                                   |                                                                                      
agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc                                                                  <  2:266432/90‑1 (MQ=255)
    tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGCTGcg                                                              >  1:624205/1‑90 (MQ=255)
     cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgt                                                             <  1:292298/90‑1 (MQ=255)
     cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgt                                                             <  2:627770/90‑1 (MQ=255)
               aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc                                                   <  1:636541/90‑1 (MQ=255)
                 gggTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCGGGTTTTTTGGTGCGTCGTACTGAActt                                                 >  1:450142/1‑90 (MQ=255)
                                            cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTCGCTGCGTCGGACTGACCTTCTTTTTACAACACCCGGAGCGgcgcgc                      >  1:219958/1‑90 (MQ=255)
                                            cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCCGGAGCGTCgcgc                      >  2:312333/1‑90 (MQ=255)
                                            cctcAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTTCGCTGCGTCGGACTGACCTTTTTTTTACAGCACCCGGAGCGTCgcgc                      >  1:189248/1‑90 (MQ=255)
                                                 aGCATTTCATTGGCGGGGGGGGGGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTTACAGCACCGGGAGCGTCGCGCccaca                 >  1:286156/1‑90 (MQ=255)
                                                      ttCATTGGCGGGGGGGGGGGGGAGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCCGGAGCGGCGCGCCAacaacaaa            >  1:274272/1‑88 (MQ=255)
                                                      ttCATTGGCGGGGGGGGGGGCGGGCAGTTTTTTTGCTGCGTGGTTCTGACCTTCTTTTTAAAACACCCGGGGCGTCGCGCCCACAACATa            >  2:318920/1‑90 (MQ=255)
                                                                gggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTg  <  2:2311/90‑1 (MQ=255)
                                                                   |                                                                                      
AGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTG  >  NZ_CP009273/585319‑585472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑