| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 585,390 | 0 | T | G | 57.1% | 4.7 / 15.7 | 14 | T341P (ACC→CCC) | nfrA | bacteriophage adsorption protein NfrA |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGG > NZ_CP009273/585319‑585476 | agCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTgcgc < 2:266432/90‑1 (MQ=255) tCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGGAGGTTTTTTGCTGcg > 1:624205/1‑90 (MQ=255) cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgt < 1:292298/90‑1 (MQ=255) cAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGcgt < 2:627770/90‑1 (MQ=255) aCGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGAcc < 1:636541/90‑1 (MQ=255) gggTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGGGCGGGTTTTTTGGTGCGTCGTACTGAActt > 1:450142/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGTTTTTTCGCTGCGTCGGACTGACCTTCTTTTTACAACACCCGGAGCGgcgcgc > 1:219958/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGGGGGCAGGTTTTTTGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCCGGAGCGTCgcgc > 2:312333/1‑90 (MQ=255) cctcAAGCATTTCATTGGCGGGGGGGGGGGCGAGCAGGTTTTTCGCTGCGTCGGACTGACCTTTTTTTTACAGCACCCGGAGCGTCgcgc > 1:189248/1‑90 (MQ=255) aGCATTTCATTGGCGGGGGGGGGGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTTACAGCACCGGGAGCGTCGCGCccaca > 1:286156/1‑90 (MQ=255) ttCATTGGCGGGGGGGGGGGGGAGCAGTTTTTTCGCTGCGTCGTACTGACCTTCTTTTTAAAGCACCCGGAGCGGCGCGCCAacaacaaa > 1:274272/1‑88 (MQ=255) ttCATTGGCGGGGGGGGGGGCGGGCAGTTTTTTTGCTGCGTGGTTCTGACCTTCTTTTTAAAACACCCGGGGCGTCGCGCCCACAACATa > 2:318920/1‑90 (MQ=255) gggAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTg < 2:2311/90‑1 (MQ=255) gggTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCgg > 2:246618/1‑90 (MQ=255) | AGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCAGTTTTTGCGCTGCGTCGTACTGACCTTCTTTTAACAGCACCGGTAGCGTCGCGCCAACAACATACTGGCGGTTGTCGG > NZ_CP009273/585319‑585476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |