| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,530,046 | 0 | C | A | 55.6% | 4.4 / 11.9 | 9 | V24F (GTT→TTT) | yddE | PhzF family isomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base A (5/0); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACG > NZ_CP009273/1529970‑1530120 | aaaGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCCGaa > 1:681533/1‑90 (MQ=255) ggCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACCGCAGAAATGCCGCGGAAAgggt > 2:65267/1‑88 (MQ=255) gcgGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTaaa < 2:475432/90‑1 (MQ=255) gATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATc < 1:384868/90‑1 (MQ=255) gATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATc < 1:65267/90‑1 (MQ=255) gCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGt < 2:681533/90‑1 (MQ=255) tGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAACACCCGCAGAAATGCCGCGGAAAGGGTGTGGGGGAAAAAAAACGACgggg > 1:543984/1‑90 (MQ=255) ttCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAAATGCCGCGAAAAGGGTGTGGGGGAAAAAAAACGACGGGGTACACCCGCgg > 1:717731/1‑90 (MQ=255) tATCGGCGGGGGAAAAAAAACCCGCAGAATTGCCGCGAAAAGGGTGTGGGGGAAAAAAATCGACGGGGGGCACCCGCGGGTTTAtttaag > 1:481010/1‑86 (MQ=255) | AAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACG > NZ_CP009273/1529970‑1530120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |