| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,622,316 | 0 | T | G | 57.1% | 3.3 / 11.3 | 14 | G181G (GGT→GGG) | ydfG | bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG > NZ_CP009273/1622227‑1622394 | gCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTggtgggt < 2:54653/90‑1 (MQ=255)gCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTggtgggt < 2:590856/90‑1 (MQ=255) tGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTcc < 1:442720/90‑1 (MQ=255) gCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCGCTTTaa > 2:215113/1‑90 (MQ=255) gCGTACGGATCTGCATGGTACGGCGGTGCGCGGCACCCACATCGGACCCGGGCTGGGGGGGGGGACCGGGGTTTTCAATGGCCGCGTTaa > 2:1393/1‑90 (MQ=255) tACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAgg < 1:128269/90‑1 (MQ=255) aTCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGTGGGGGGGACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATg > 1:487720/1‑90 (MQ=255) cTGCATGGTACGGCGGTGCGCGGCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCGCTTTTAAGGCGATGAc > 2:434366/1‑90 (MQ=255) gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCAAAGTCCGCTTTTAAAGCGATGACGGGAAAg > 2:329228/1‑90 (MQ=255) gTGCGCGTCACCGACATCGAACCGGGTCTGGGGGGGGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGaaaaa > 1:541588/1‑90 (MQ=255) gTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGaaaaa > 2:572787/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc > 1:686328/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGTGGGTGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAGCAGAAAAAAc > 1:654385/1‑90 (MQ=255) gcgcGTCACCCACATCTAACCGGGGCTGGTGGGGGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc > 2:572760/1‑90 (MQ=255) ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg < 1:42345/90‑1 (MQ=255) ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg < 2:487720/90‑1 (MQ=255) | GCGTTTGTTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG > NZ_CP009273/1622227‑1622394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |