Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,622,319	0	T	G	57.1%	6.4 / 13.0	14	G182G (GGT→GGG)	ydfG	bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (8/0);  total (10/4)
Fisher's exact test for biased strand distribution p-value = 1.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.11e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG  >  NZ_CP009273/1622249‑1622394                                                                       |                                                                            tGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTcc                                                          <  1:442720/90‑1 (MQ=255)        gCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCGCTTTaa                                                   >  2:215113/1‑90 (MQ=255)        gCGTACGGATCTGCATGGTACGGCGGTGCGCGGCACCCACATCGGACCCGGGCTGGGGGGGGGGACCGGGGTTTTCAATGGCCGCGTTaa                                                   >  2:1393/1‑90 (MQ=255)           tACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAgg                                                <  1:128269/90‑1 (MQ=255)                aTCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGTGGGGGGGACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATg                                           >  1:487720/1‑90 (MQ=255)                  cTGCATGGTACGGCGGTGCGCGGCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTTCAATGTCCGCTTTTAAGGCGATGAc                                         >  2:434366/1‑90 (MQ=255)                         gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCAAAGTCCGCTTTTAAAGCGATGACGGGAAAg                                  >  2:329228/1‑90 (MQ=255)                                 gTGCGCGTCACCGACATCGAACCGGGTCTGGGGGGGGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGaaaaa                          >  1:541588/1‑90 (MQ=255)                                 gTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGaaaaa                          >  2:572787/1‑90 (MQ=255)                                   gcgcGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc                        >  1:686328/1‑90 (MQ=255)                                   gcgcGTCACCGACATCGAACCGGGTCTGGTGGGTGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAGCAGAAAAAAc                        >  1:654385/1‑90 (MQ=255)                                   gcgcGTCACCCACATCTAACCGGGGCTGGTGGGGGGGACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc                        >  2:572760/1‑90 (MQ=255)                                                         ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg  <  1:42345/90‑1 (MQ=255)                                                         ggTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTg  <  2:487720/90‑1 (MQ=255)                                                                       |                                                                            TGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTG  >  NZ_CP009273/1622249‑1622394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑