| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,002,571 | 0 | A | G | 57.1% | 5.4 / 11.9 | 14 | I272V (ATC→GTC) | yedE | selenium metabolism membrane protein YedE/FdhT |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACT > NZ_CP009273/2002482‑2002647 | ttACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGC‑GGTGAGTGCCATCGGGa < 1:35153/90‑1 (MQ=255) aGCGTTCCGCGATATGTGGATCACCGGACGTA‑CCATATGGCCGAAGGAATCATTTTTGGGATGGCGGGTGAGGGCCACCGGGATTTTCAg > 1:386384/1‑90 (MQ=255) ccGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGC‑GGGGAGTGCCCTCGGGGTCTTCAGTTACGTACAGTTAGGGGGTGAAc > 2:193928/1‑90 (MQ=255) cGTACCCATATGGCGAAAGCAATCATTATCGGTATGGC‑GGGGGGGGCCCCCCGGGGCTTCCGTTAACTAAAGTTTGGCTTTGAAACCcaa > 2:478668/1‑90 (MQ=255) atatGGCGAAAGCAATCATTATCGGTATGGC‑GGTGGGGGCCATCGGGGTCTTCAGTTACGTACAGTTAGGGGGTGAAACCAAAATCAtgt > 1:649173/1‑90 (MQ=255) gCGAAAGCAATCATTATCGGTATGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCg > 1:212409/1‑90 (MQ=255) gCGAAAGCAATCATTATCGGTATGGC‑GGTGAGGGCCATCGGGGTCTTCCGTTACGTACAGTTAGGGGTTTAAACCAAAAACATGTggggg > 2:648818/1‑88 (MQ=255) gCGAAAGCAATCATTATCGGTATGGC‑GGGGAGTGCCCTCGGGGTCTTCAGTTACCTACAAGTAGGGGTTTAACCCCAAAACATGGGGGCg > 2:212588/1‑90 (MQ=255) gCGAAAGCAATCATTATCGGTATGGC‑GGGGAGTGCCCTCCGGGTCTTCAGTTTAGTACAGTTAGGGGTTTAACCCAAAATTATTTGGGCg > 2:697461/1‑90 (MQ=255) gCAATCATTATCGGTATGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCa < 2:634464/90‑1 (MQ=255) ttATCGGTATGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCgg < 2:60852/90‑1 (MQ=255) ggTATGGC‑GGTGAGTGCCAACGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAAtt < 2:470815/90‑1 (MQ=255) aTGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATtggt < 1:396796/90‑1 (MQ=255) gAGTGCCCTCGGGGTCTTCAGTTACGTAAAGTTAGGCGGTGAAACCAAAAACATGTGGGGGGGGCCAAACGCGGGAATTGGGGGGTTTCt > 2:563220/1‑90 (MQ=255) gAGTGCCATCGGGGTCTTTAGTTACGTACAGTTAGGCGTTGAACCCAAAAACATGTGGGGGGGGCCAAACGCGGGAATTGGGGGGTTTCt > 2:186526/1‑90 (MQ=255) | TTACTTCAGCGTTCCGCGATATGTGGATCACCGGACGTACCCATATGGCGAAAGCAATCATTATCGGTATGGC‑GGTGAGTGCCATCGGGATCTTCAGTTACGTACAGTTAGGCGTTGAACCCAAAATCATGTGGGCGGGACCAAACGCGGTAATTGGTGGTTTACT > NZ_CP009273/2002482‑2002647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |