| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,255,305 | 0 | T | G | 57.9% | 17.4 / 21.4 | 20 | A180A (GCA→GCC) | fruK | 1‑phosphofructokinase |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (11/0); total (13/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.59e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTG > NZ_CP009273/2255220‑2255367 | cAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCg < 1:544312/90‑1 (MQ=255) ttCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGGTAGGGTTTACCCACCCCGGGGCCGCttt > 2:639821/1‑90 (MQ=255) caTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCTTTCAAACCTg > 1:686964/1‑90 (MQ=255) tttcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCCGCCACGGGGCCGCTTTTAAAACTGctac > 2:151093/1‑90 (MQ=255) ttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTTTGTTTCCCCCGCCCCGGGGCCCCCTTTAAACCTGCTACTaaacc > 2:466374/1‑87 (MQ=255) ggCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCa < 1:154556/90‑1 (MQ=255) tttACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGct < 1:466374/90‑1 (MQ=255) tACGGCCTGCCCAGATTTCCAGCTCGCGGCTGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGctac < 2:90368/90‑1 (MQ=255) tACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGctac < 2:232521/90‑1 (MQ=255) tACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGctac < 1:151093/90‑1 (MQ=255) tGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTTACCAGCCCCGGGGCCCCTTTCAAACCTGCTACTAACGCTTCACGGCCACTATCaaa > 2:308505/1‑90 (MQ=255) tGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTTACCAGCCCCGGGGCCGCCTTCAAAACCGCCACTAACGCCTCCCGGCTACTATCaaa > 2:628448/1‑90 (MQ=255) tGCCCAGATTTCCAGCTCGCGGCGGTTAGGGTTCACCAGCCACGGGGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCaaa > 1:507087/1‑90 (MQ=255) tGCCCAGATTTCCAGCTCGCGGCGGGTAGGTTTTACCAGCCCCGGGGCCCCCTTCAAACCCGCTCCTAACGCTTCCCGGGTACTATCaaa > 2:467202/1‑90 (MQ=255) gCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAg > 1:1491/1‑90 (MQ=255) gATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGGGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAAt > 2:718386/1‑90 (MQ=255) gATTTCCAGCTCGCGGCGGTTAGGGTTCACCAGCCACGGCGCCGCTTTCAAACCTGCTGCTAACGCTTCACGGCTCCTATCAAAaataat > 1:462062/1‑90 (MQ=255) tCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGacac < 2:450795/90‑1 (MQ=255) tCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCCCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGacac > 1:618426/1‑90 (MQ=255) gcggcgGTTAGGGTTCACCAGCCACGGGGCCGCTTTCAAAACTGCTACTAACGCTTTACGGCTACTATCAAAGATAATGCAAAGacaccg > 1:486804/1‑88 (MQ=255) | CAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTG > NZ_CP009273/2255220‑2255367 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |