| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 56.2% | 3.8 / 17.0 | 16 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base T (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGT > NZ_CP009273/3175871‑3176041 | cGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGGGCGACGGTTAttt > 2:668789/1‑89 (MQ=255) aaGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTTTTGGCGCGGTTTTCcgcggt > 1:557128/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACCGTTTTTTGTGCGGTTTTCCGCGGTCTCGGGCcaaaaac > 2:638281/1‑88 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGCGCGTTTTTCGGCGTTCTCGGGCAAAAAACCTCGAAcc > 1:44042/1‑90 (MQ=255) gTACCTCTCATTCTGACCATACTGGCGGGGGGGGCCACGTTTTTTGGCGCGGTTTTCCGCGGTCTCGGGCAAAAACCCCCCCACCGCttt > 2:704524/1‑89 (MQ=255) ccTCTCATTCTGACCATACTGGCGGGGGCCGCCACGTTTTTTGGGGCGTTTTTCCGCGTTCTCGGGCAAAAACCCCCCCACCCCTTACTg > 2:16453/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTTGCGCGGTTTTCCGCGTTCTCCGGCAAAAAACCCCCAACCCCTTACTggg > 2:204183/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCACGGGTTTTGGTGGGGTTTTTGGCGGTCTCCGGCGAAAAACCCCCAACCGCGTATTggg > 2:428398/1‑89 (MQ=255) cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGtt < 2:487849/90‑1 (MQ=255) cTGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGTTTCTCCGCGTTCTCCGGCAAAAACCCCCCAACCGCTTACTGGCGgttttg > 1:244772/1‑88 (MQ=255) tGACCATACTGGCGGGGGCAGCCACGTTTTTTGGCGCGGTTCTCCGCGTTCTCGGGCAAAAAACCCCGAACCCCCTTCTGGGGGTTTTGc > 1:5254/1‑90 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:502827/90‑1 (MQ=255) ggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggcg < 1:344544/90‑1 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:435127/90‑1 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 2:88650/90‑1 (MQ=255) agTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGGTCATGt > 1:618512/2‑90 (MQ=255) | CGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCTCGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGATCATGT > NZ_CP009273/3175871‑3176041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |