Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,725,0840GA66.7% ‑5.8 / 17.6 24L198L (TTG→TTA) xylFD‑xylose ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base A (0/16);  total (6/18)
Fisher's exact test for biased strand distribution p-value = 2.08e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGC  >  NZ_CP009273/3725011‑3725167
                                                                         |                                                                                   
cTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAAcg                                                                     >  2:531240/1‑90 (MQ=255)
cTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAAcg                                                                     >  1:376447/1‑90 (MQ=255)
         aTTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCg                                                            >  1:739793/1‑90 (MQ=255)
            ccGAAAAAATAAAAGTCGTTGGTGACAAATGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTAGGAAAAACGCGCTAACCGCCa                                                         <  2:34211/90‑1 (MQ=255)
               gAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCaata                                                      <  1:594735/90‑1 (MQ=255)
                     taaaaGCCGTTGGTGACCAATGGGTTGATGGCTGGTTCCCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACa                                                <  1:40998/88‑1 (MQ=255)
                          tccGTTGGTGCCCATTGGGTTGTGGGCTGGTTACGGGAAAACCCATTAAAATTTTTGGAAACCGGGCCATCCGCCAATAATAACAAAAtt                                           <  2:733414/88‑1 (MQ=255)
                          gTCGTTGGTGGCCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTAAAAATTATGGAAAACGCCCTAACCGCCAATAATAACAAAAtt                                           <  1:363157/90‑1 (MQ=255)
                          gTCGTTGGTGACCAATGGGTTGATGGCTGGTTACGGAAAAACGCATTAAAAATTAGGAAAAACGCGATAACCGCCAATAATAACAAAAtt                                           <  2:346923/90‑1 (MQ=255)
                          gTCGTTGGTGACAAAGGGGTTGATGGCTGGTTACCGAAAAACGAATTAAAAATTATGAAAACCGCGATAACCGCCAATAATAACAAAAtt                                           <  2:712732/90‑1 (MQ=255)
                             gTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGAt                                        >  1:326058/1‑90 (MQ=255)
                             gTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGAt                                        <  1:293100/90‑1 (MQ=255)
                                    ccaaaTGGGTTGATGGCTGGTTACCGAAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAg                                 <  1:339071/87‑1 (MQ=255)
                                           ggTTGATGGCTGTTTACCGAAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTc                          <  2:266008/90‑1 (MQ=255)
                                               gATGGCTGGTTCCGGAAAAACGCATTAAAATTTATGAAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAAc                      <  1:755554/90‑1 (MQ=255)
                                               gATGGCTGGTTACGGAAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAAc                      <  2:25843/90‑1 (MQ=255)
                                               gATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAAc                      >  1:266008/1‑90 (MQ=255)
                                                      ggTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCa               >  2:535108/1‑90 (MQ=255)
                                                      ggTTACCGAAAAACGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCa               <  1:309145/90‑1 (MQ=255)
                                                         tACCGAAAAACGAATTAAAAATTATGAAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCg            <  1:636252/90‑1 (MQ=255)
                                                          cccGGAAAACGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGc           <  1:615325/89‑1 (MQ=255)
                                                                   cGCATTAAAAATTATGGAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGc  <  2:156269/90‑1 (MQ=255)
                                                                   cGCATTAAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGc  <  1:72143/90‑1 (MQ=255)
                                                                   cGCATTAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGc  <  2:215836/90‑1 (MQ=255)
                                                                         |                                                                                   
CTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTTGATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGC  >  NZ_CP009273/3725011‑3725167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑