| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 5,159 | 0 | G | A | 57.9% | ‑0.0 / 20.2 | 19 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base A (0/11); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5078‑5243 | tGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATaacaa > 1:201212/1‑90 (MQ=255) tGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTtctc > 1:438144/1‑90 (MQ=255) gaaaaaaaaTAAAAGGAAAAAAGGAAAAATTCTAAAAAAAGGGGGTAATTTAGAAATTGGGTTGGGGAAAAAAAACAACCGCCGTTCTCa < 2:320977/86‑1 (MQ=255) aaaGGACAGGAAAAAAGAAAAAATTCTAAAAAAATGGGGTAATTTAGAGATTAGGATGGGGGAAAAAAACACCCGCCGTTCTCATCGAGt < 2:170018/90‑1 (MQ=255) aaTGACAGGAAAAAAGGAAAAATTCTCAAAAAATGCGGTAACTTAGAGATTAGGATTGCGGAAAATAACAACCGCCGTTCTCATCGAGTa < 1:183272/90‑1 (MQ=255) tGAAAGGAAAAAAGAAAAAATTCAAAAAAAAGGGGTTAATTTAGAGTTTGGGATGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAAt < 2:431913/90‑1 (MQ=255) cggaaaaaaGGAAAATTCCACAATAAACGCGGTACTTTAGAAATTAGGATTCCGGAAAATAACAACCGCCGTTCTCATCGAGTAATCTcc < 1:178219/86‑1 (MQ=255) gaaaaaTTCTCAAAAATGGGGGTAACTAAGAGATTGGGTTGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAc < 2:223341/87‑1 (MQ=255) agaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcc > 2:333797/1‑90 (MQ=255) aaaTCCTCAAAAAATGCGGTAATTTAGAGATTAGGATTGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCa < 2:678208/90‑1 (MQ=255) ttCTAAAAAAAGGCGGTAACTTAGAGATTAGGATTGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATaa < 1:429935/90‑1 (MQ=255) aaaaaaTGCGTTAATTAAGAGATTAGGGGGGGGGAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGc < 2:538191/87‑1 (MQ=255) tAAAGGCGGTAACTTAGAGATAAGGTTGGGGAAAAAAAAAAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 1:364373/90‑1 (MQ=255) aaaaGGCGTTAATTAAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCaa < 2:89178/89‑1 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 2:535707/1‑90 (MQ=255) ttAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgt > 1:258489/1‑90 (MQ=255) tAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCtgtg > 1:46188/1‑90 (MQ=255) gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa > 1:682597/1‑90 (MQ=255) gCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGa > 1:349669/1‑90 (MQ=255) | TGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGA > NZ_CP009273/5078‑5243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |