| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,178,250 | 0 | A | C | 56.2% | 4.4 / 12.8 | 16 | G12G (GGT→GGG) | potD | spermidine/putrescine ABC transporter substrate‑binding protein PotD |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCA > NZ_CP009273/1178163‑1178339 | tCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCAcc < 1:112077/90‑1 (MQ=255) cACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAg > 2:427213/1‑90 (MQ=255) ccGTCCTCGGCCCAGTTGTAAAAATACGGGGTGTTGTTGTCATCGGCGGGGGCGGCGCTCATGCCCAGTGCAAGAGCCCCCGCCGCGAGc < 2:478857/89‑1 (MQ=255) ggTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCg > 1:149598/1‑90 (MQ=255) gTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGt > 2:215889/1‑90 (MQ=255) cGTGTTGTTGTCAGCGGGGTGGGGGGCGCTCATCCCAAGTACCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACg < 2:530524/90‑1 (MQ=255) ttgttgtCAGCGGGGTGAGCGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTccc < 2:197803/90‑1 (MQ=255) ttttgtCATCGGCGGGGGGGGCGCTCATGCCCAGTGCCAGACCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTcccc < 2:75385/88‑1 (MQ=255) tgttgtCATCGGGGTGGGCGGCCCTCCTGCCCAGTGCAAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTcccc < 2:413096/90‑1 (MQ=255) cATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGtt > 1:377191/1‑90 (MQ=255) cATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGGGGCGTGGCCCTTTTTTCATTTAACGTCCCCTGGGtt > 2:101047/1‑90 (MQ=255) cGTGGGGGGCGCTCATGCCCAGTGCCAGAGCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCttt < 1:513105/90‑1 (MQ=255) gcggcgCTCTTCCCCAGTCCCAGCCCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGttt < 2:86653/90‑1 (MQ=255) cATGCCCAGTCCCAGACCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGa < 2:149598/90‑1 (MQ=255) gagCCCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggct < 1:336704/90‑1 (MQ=255) gagCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAgctggct > 1:310528/1‑90 (MQ=255) aCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGa > 2:137563/1‑72 (MQ=255) aCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGa < 1:137563/72‑1 (MQ=255) aCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa > 1:386745/1‑90 (MQ=255) aCCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCa > 1:375535/1‑90 (MQ=255) | TCCTGGCGGCACGTACTCGGTCCAGTTGTAGAAATACAGCGTGTTGTTGTCATCGGCGTGAGCGGCGCTCATGCCCAGTGCCAGAGCACCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCA > NZ_CP009273/1178163‑1178339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |