| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,089,571 | 0 | A | G | 60.0% | 2.0 / 11.8 | 15 | I83V (ATT→GTT) | hisF | imidazole glycerol phosphate synthase subunit HisF |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (9/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTA > NZ_CP009273/2089485‑2089660 | cTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTa < 2:291707/90‑1 (MQ=255) tCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGGAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAg < 1:311856/90‑1 (MQ=255) ccGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGGCTCTGGAAGa > 2:438122/1‑90 (MQ=255) gCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGGCTTTGGGAGAAGGCGCGAAAATTAttt > 2:557492/1‑90 (MQ=255) gCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAAATtttt > 1:206064/1‑90 (MQ=255) tGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAAGCTCTGGAAGAGGGCGCGGAAAATCttttc > 2:28099/1‑88 (MQ=255) gggTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGGTAAGTCTCTGGGAGATGTTGCCGAAATTCTTTCCt > 1:357438/1‑90 (MQ=255) tctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTgc > 1:157992/1‑90 (MQ=255) tctcGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGGGGGGTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTTCTTTTgc > 1:525959/1‑90 (MQ=255) cgcgTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGcgcg < 1:188698/90‑1 (MQ=255) gACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAAc < 1:567972/90‑1 (MQ=255) gTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCCCGAAAATTTTTTCCTTTGGCGCGGATAAAAATTCCATCAACTCTCCCgc > 1:204829/1‑90 (MQ=255) ttGTGTGGCGGGGGGGGTTAAATCTCTGGAAGATGCCGCGAAAATTTTTTTCTTTTGCGCGGGTAAAATTTTCATCAACTCTCCCGCGCt > 2:133851/1‑90 (MQ=255) gtgtgGCGGGGGGGGTTAAGTCTCTGGAAGATGCCCCGAAAAATTTTTTCTTTGGGGCGGGTAAAAATTTCATCAAATCTCCTGCGCTgg > 2:24338/1‑90 (MQ=255) gCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGAc < 1:60782/90‑1 (MQ=255) aTTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACatta < 2:131223/90‑1 (MQ=255) | CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCGGGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCTGGCAGACCCAACATTA > NZ_CP009273/2089485‑2089660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |