Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,402,2930GT54.5% 7.2 / 15.5 11intergenic (+36/‑48)alaA/yfbRalanine transaminase AlaA/5'‑deoxynucleotidase
Reads supporting (aligned to +/- strand):  ref base G (5/0);  new base T (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTAATCTTAATTT‑CACTGCCGGAGATTGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTC  >  NZ_CP009273/2402209‑2402370
                                                                                     |                                                                             
ttttttGAACAAGTCCGCGCTTTTCTTTTTTGTTTATCACCAGTTATATTTTTATTTT‑CACTGCCGGAGATTGCACCCGGCAGCTTTATc                                                                          <  2:241575/87‑1 (MQ=255)
 tttttGAGCAAGTTCGCGCTTTTCTTTTCTGGTTATCCTCAGCT‑TTATTTTATTTT‑CCCTGCCGGAGATTGAACCCGGCGGCTTTATccc                                                                        <  2:587516/88‑1 (MQ=255)
          aaGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTAATCTTAATTT‑CACTGCCGGAGATTGCATCCGGCAGCGTTATCCCGCCACAAt                                                                >  1:404210/1‑90 (MQ=255)
          aaGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTAATCTTAATTT‑CACTGCCGGAGATTGCATCCGGCAGCGTTATCCCGCCACAAt                                                                >  2:397191/1‑90 (MQ=255)
                  gagTTTCCTTTCTGTTTACCATAAGCTGT‑ATTTTAATTTCCACTGCCGGAGATTGCGTCCGGCAGCTTTCTCCCGCCACAATGACCtgat                                                        <  2:461466/88‑1 (MQ=255)
                                       tcaGTTGTAATTTTATTTT‑CTCTGCGGGAGTTTCCACCCGGCGGCTTTACCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTc                                   <  2:538677/90‑1 (MQ=255)
                                       tcaGCTTTAATCTTATTTT‑CACTGCGGGAGATTGCACCCGGCGGCTTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTc                                   <  2:544705/90‑1 (MQ=255)
                                       tcaGCTGTAATCTTAATTT‑CCCTGCCGGAGATTGCACCCGGCAGCTTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTc                                   <  2:218013/90‑1 (MQ=255)
                                                    tAATTT‑CACTGCCGGAGATTGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTCACTATGAAACaga                      >  1:514647/1‑90 (MQ=255)
                                                                         gCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCAtctc  >  1:218886/1‑90 (MQ=255)
                                                                         gCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCAtctc  >  2:43013/1‑90 (MQ=255)
                                                                                     |                                                                             
GTCTTTGAGCAAGTTCGCGCGTTTCCTTTCTGGTTATCATCAGCTGTAATCTTAATTT‑CACTGCCGGAGATTGCATCCGGCAGCGTTATCCCGCCACAATGACCTGATGATGTCATCATACGTAAGGTCACTATGAAACAGAGCCATTTTTTTGCCCATCTC  >  NZ_CP009273/2402209‑2402370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑