| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,828,692 | 0 | A | C | 58.3% | 7.1 / 12.5 | 12 | V698G (GTT→GGT) | hypF | carbamoyltransferase HypF |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTCTGTGGAAAGAGCAATGTGAAATCAGCGAGATAATGCGCCAGACGTGCACGCAGCAAACGGTTATGAATAACCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGCATCATGA > NZ_CP009273/2828618‑2828781 | aaCTCTGTGGAAAGAGCAATGTGAAATCAGCGAGATAATGCGCCAGACGTGCACGCAGCAAACGGTTATGAATAACCCCGCCGCTAAATa > 2:442892/1‑90 (MQ=255) cctctGGGAAAAAAGAAATGAAAAATCAGCGAAATAAGGCCCCAGCCGGCCCCGCAGAAAACGTTTATAAATACCCCCGCCGCTAAATAc < 1:378583/89‑1 (MQ=255) tgtgAAAAGAGCAATGTAAAATCAGCGAAATAATGCGCCAGACGTGCACGCAGAAAACGTTTATAAATACCCCCGCCGCTAAATACCAGc < 1:233603/90‑1 (MQ=255) gtgGAAAGAGCAATGTGAAATCAGCGAGATAATGCGCCAGACGTGCACGCAGCAAACGGTTATGAATAACCCCGCCGCTAAATACCAGCg > 2:347397/1‑90 (MQ=255) ggaaaaGAGAAATGAAAAACCAGCAAAATAAGGCCCCAGACGCGCACGCAGCAAAGGTTTATAAATACCCCCGCCGCTAAATACCAGCGt < 1:347397/87‑1 (MQ=255) gaaaaaAGAAATGTAAAACAAGCGAAAAAAGGCGCCAGCCGGCCCCGAAGAAAACGTTTATAAATACCCCCGCCGCTAAATACCAGCGTa < 2:250388/88‑1 (MQ=255) tgaaaaaTCAGCAAAAAAAGGCCCCAGCCGGCCACGCAGAAAAGGTTTATAAATACCCCCGCCGCTAAATACCAGCGTAGTGATACCACg < 1:442892/86‑1 (MQ=255) aTAATGCGCCGGCGGTCCACGCAGAAAGCGTTTAAAAAAACCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTc < 1:506149/90‑1 (MQ=255) aTAAGGCCCCAGCCGTGCCCGCAAAAAACGTTTAAAAATACCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTc < 1:374666/90‑1 (MQ=255) cagcaAACGGTTATGAATAACCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAAcc > 2:557082/1‑90 (MQ=255) cagcaAACGGTTATGAATAACCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGACTGCTCACGCATCAACGCGGCAAAAcc > 2:425703/1‑90 (MQ=255) tAACCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGcatcat > 2:438565/1‑90 (MQ=255) aCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGcc > 2:139051/1‑45 (MQ=255) aCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGcc < 1:139051/45‑1 (MQ=255) aCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGCATCATGa > 2:477894/1‑90 (MQ=255) aCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGCATCATGa > 2:532178/1‑90 (MQ=255) aCCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGCATCATGa > 2:562803/1‑90 (MQ=255) | AACTCTGTGGAAAGAGCAATGTGAAATCAGCGAGATAATGCGCCAGACGTGCACGCAGCAAACGGTTATGAATAACCCCGCCGCTAAATACCAGCGTAGTGATACCACGCATCGTGGCCTGCTCACGCATCAACGCGGCAAAACCCTGCGCCAGCGCATCATGA > NZ_CP009273/2828618‑2828781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |