| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,852,466 | 0 | T | G | 58.8% | 3.7 / 15.9 | 17 | V672G (GTG→GGG) | mutS | DNA mismatch repair protein MutS |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base G (0/10); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852377‑2852555 | acaAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTAtggt > 2:126222/1‑90 (MQ=255) aaGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGgaga < 1:101471/90‑1 (MQ=255) aaGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGgaga < 2:442230/90‑1 (MQ=255) gTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATg > 1:551033/1‑90 (MQ=255) gACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCg > 1:137942/1‑90 (MQ=255) tATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCaa > 1:100318/1‑90 (MQ=255) tcgCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATAtttt > 1:242220/1‑90 (MQ=255) tccccGGGTGGGCGCGGAAGAGGCCCGGGCGCCCGGGCCCTCACCT‑TTTGTGGGGGGGAGGACTGAAACCGCCAATATTTTACATAACGc < 2:147129/88‑1 (MQ=255) ccGCGTGGGCGGGGCAGATGACCTGGCGTCCGGGCCCCCACCC‑TTTAGGGGGGAGATGATTAAAACCGCCAATATTTTACATAACGCCAc < 1:218660/90‑1 (MQ=255) gggggcgGCAGATGCCCGGGCGCCCGGGCCCCCAACC‑TTTAGGGGGGAGATGAATAAAACCGCCAATATTTTACATAACGCCACCGAATa < 2:551033/86‑1 (MQ=255) ggCGCGGCAGATGCCCGGGCG‑CCGGGGCCCCAACCTTTTAGGGGGGAGATGAGTAAAACCGCCAATATTTTACATAACGCCACCGAATAc < 2:149247/90‑1 (MQ=255) ggCAGATGACCTGGCGCCCGGGCGCTCACCT‑TTTATGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCt < 1:32526/90‑1 (MQ=255) cAGATGCCCGGGCGTCGGGGCGCCCACCC‑TTTAGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTgg < 2:352095/90‑1 (MQ=255) tGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATgg > 2:416765/1‑90 (MQ=255) cGTCCGGGCGCCCACCC‑TTTATGGGGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATg < 1:141354/90‑1 (MQ=255) gggCGCTCACCC‑TTTATGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 1:197752/90‑1 (MQ=255) gggCGCTAAACT‑TTTGTGGGGGAGAGGATTAAACCCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATc < 2:137942/90‑1 (MQ=255) ccAACT‑TTTGGGGGGGAGATGACTAAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGt < 2:249191/89‑1 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 2:514957/1‑90 (MQ=255) tggAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCt > 1:216314/1‑90 (MQ=255) | ACAAAAAGTCGAGATTGGACCTATCGATCGCATCTTTACCCGCGTAGGCGCGGCAGATGACCTGGCGTCCGGGCGCTCAACC‑TTTATGGTGGAGATGACTGAAACCGCCAATATTTTACATAACGCCACCGAATACAGTCTGGTGTTAATGGATGAGATCGGGCGTGGAACGTCCACCT > NZ_CP009273/2852377‑2852555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |