| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,090,826 | 0 | T | G | 58.8% | 10.5 / 14.1 | 17 | G67G (GGT→GGG) | hemW | radical SAM family heme chaperone HemW |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (10/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAG > NZ_CP009273/3090748‑3090908 | gCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCGAGccc > 1:478841/1‑89 (MQ=255) tGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGGCCCCGAGCCGGCttt > 1:431971/1‑90 (MQ=255) aCGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCCGCTTTTCCGCCCCGCGGTGCaaa > 2:252982/1‑90 (MQ=255) aCGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGATGCaaa > 1:67826/1‑90 (MQ=255) cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc < 1:501877/90‑1 (MQ=255) cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc < 1:307709/90‑1 (MQ=255) cGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAc < 2:163748/90‑1 (MQ=255) cGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTTCCGCCCCGCGATGCAAACCCTGCTGGGCgg > 1:410001/1‑90 (MQ=255) cTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGgcg < 1:313002/90‑1 (MQ=255) cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGGCGGGGCCGCTTTTCGGCCCCGGGGTGCCAAAGGCGCTGGAggggggg > 2:39461/1‑86 (MQ=255) cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGGTGCAAACGCTGCTGGGCGgcgtg > 1:94530/1‑90 (MQ=255) cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGGACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAAAGCTGCTGGACGgcgtg > 1:63130/1‑90 (MQ=255) cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGGCGCCGAGGCGGCTTTTCCGCCCCGCGATGCAAACACTGCTGGGCgggggg > 2:352419/1‑86 (MQ=255) cAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGGACGCCCGGCCCGCTTTTCCGCCCCGCGGTGCAAAACCCGCCGGgcggcggg > 2:130582/1‑88 (MQ=255) aGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGGGGTACCCCCAGCCCCCTTTTCGGCCCCGCGCTGCAAAAGCTCCTGGGGGgcgggg > 2:463114/1‑87 (MQ=255) tCTTTATTGGCGGTGGGACGCCGCGCCTGCTTTCCCGCCCCGCGATGCAAAAGCCGCTGGGCGGCGGGGGGGCGCGGTTGCCGCTggggg > 2:218763/1‑87 (MQ=255) gCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAg < 2:478841/90‑1 (MQ=255) | GCATCTGCTTAACGATCTGGACAACGATGTGGCTTACGCTCAGGGCCGTGAAGTAAAGACAATCTTTATTGGCGGTGGTACGCCGAGCCTGCTTTCCGGCCCGGCGATGCAAACGCTGCTGGACGGCGTGCGTGCGCGTTTGCCGCTGGCAGCGGATGCAG > NZ_CP009273/3090748‑3090908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |