| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,118 | 0 | C | A | 53.8% | 9.9 / 23.3 | 13 | A422A (GCC→GCA) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (0/7); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCA > NZ_CP009273/3214038‑3214206 | gttgATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATc < 2:550422/90‑1 (MQ=255) tttCCCAGCGAGGTGTCCCGCCGGCGCGTCCGGGGGGCCGAAACCCTCAATAACGCAAAAACGCCCCGCATTAAACCGCcactgacactg < 1:372537/90‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:507786/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:76954/1‑90 (MQ=255) tGCCAGCGAGATGTTCCCCCAGCGCGTACTGGGGCCCGGACCGCTCAACAACGCAAAAACGACCGGCTTTGAACCGCCactgacactgac < 1:317665/90‑1 (MQ=255) tGCCAGCGAGATGTCCCGCCGGCGCGTAGTGGGGGCGGGACCGCTAAATAACGCAAAAACGACCCGCATTGAACCGCCactgacactgac < 1:359073/90‑1 (MQ=255) ccAGGGAGATTTCCCCCCGGCGCGTCGGGGGGGCGGGACCGCTAAAAAACGCAAAAACGACCCGAATTAAACCGCCACTGACACTGACCa < 1:374132/90‑1 (MQ=255) gattGTCCCGCCGGCGCGTTTGGGTGGCCGGAACGCTCAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:242167/87‑1 (MQ=255) agaTGTCCCGCCGGCGCGTGGTGGGGGCCGGACCGCTCAATACCGCAAAAACACCCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:175644/90‑1 (MQ=255) gCCAGCGGGTTCGGGTGCCCGGACCGCTAAAAAACGCAAAAACGACCCGTATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAAc < 2:395857/90‑1 (MQ=255) gcgcGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGt > 2:87154/1‑90 (MQ=255) gtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg > 2:511702/1‑90 (MQ=255) gtggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg > 2:541738/1‑90 (MQ=255) ccAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa > 2:346748/1‑90 (MQ=255) | GTTGATAACGAAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCA > NZ_CP009273/3214038‑3214206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |