| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,215,393 | 0 | A | G | 66.7% | 0.4 / 12.2 | 18 | D190G (GAT→GGT) | ebgR | transcriptional regulator EbgR |
| Reads supporting (aligned to +/- strand): ref base A (0/6); new base G (12/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCA > NZ_CP009273/3215308‑3215476 | aGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGGAGATGAACCTGGCCAGGCGGGTAtt > 2:41336/1‑90 (MQ=255) aaTCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCCGGGAAGGGGGGTATTTTTGAggg > 1:544817/1‑89 (MQ=255) atcatcGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTc < 2:498406/90‑1 (MQ=255) catcGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGGTATTTGTGAGGTCGc > 1:465978/1‑90 (MQ=255) aaCCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGGAAATGAGCCCGGCGAAGCGGGTATTTGGGAGGTTGGCTTTGGGGGAAATggg > 2:574637/1‑89 (MQ=255) aaCCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGc < 2:444583/90‑1 (MQ=255) aaCCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAAAAGAGCCTGGCAAGGGGGGTATTTGTGGGGGGGCCCTTTTGGGATATggg > 2:131029/1‑89 (MQ=255) gggCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGGGATTCGTGAGGGCGCCCTTGCGGGAAATGGGCGGCt > 2:129766/1‑90 (MQ=255) gggCGTTAATCGTATTGGTTTTATTGGCGGGGAAGAGGAGCCCGGCAAGGGGGGGATTTGTGGGGGGGCCTTTTCGGGATATGGGCGACt > 2:342542/1‑90 (MQ=255) gTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCCGGCAAAGGGGGTATTCGTGAGGGCGCCTTTTCGGGATATGGGCGACTGgaa > 1:444583/1‑90 (MQ=255) gTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGGGGGTATTTGTGGGGGGGCCTTTTCGGGATATGGGCGGCTGGAAAAAATgg > 2:54520/1‑90 (MQ=255) aTTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTa < 2:131365/90‑1 (MQ=255) ttGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGGGGGTATTTGTGAGGGCGCCCTTGCGGGATATGGCCGGCTGAAAAAAGGGGGAc > 2:469254/1‑90 (MQ=255) ggTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTAcgc < 1:351480/90‑1 (MQ=255) ttttATTGGCGGTGAAGATGAGGCTGGCAAGGCGGGTATTTGTGGGGGCGCCTTTGTGGGATATGGGCGGCTGAAAAAAGAGGGAcgcgg > 2:351480/1‑89 (MQ=255) ttttATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGa < 2:405138/90‑1 (MQ=255) tATTGGCGGTGAAGATGAGCCTGGCAAAGCGGGTATTTGTGAGGGCGCCTTTGCGGAATATGGCCGGCTGAAAAAAAGGGGACGCGAaga > 1:131365/1‑90 (MQ=255) aGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCa < 1:288436/90‑1 (MQ=255) | AGTAAAGAAATCATCGACTTCTATATCAACCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGATGAGCCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCA > NZ_CP009273/3215308‑3215476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |