| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,843,623 | 0 | A | C | 53.8% | 9.1 / 11.5 | 13 | V155G (GTG→GGG) | uhpA | transcriptional regulator UhpA |
| Reads supporting (aligned to +/- strand): ref base A (4/2); new base C (0/7); total (4/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAA > NZ_CP009273/3843535‑3843704 | gtTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCAc > 1:91403/1‑90 (MQ=255) cAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTc > 2:270615/1‑90 (MQ=255) gttgggCGGGAGGGACGTGTCCGTTTTTGGGGGACAACCCCGGTCCGGCGGCATTCTTTTTCCCCGCCATTCCTTGCGCCAGTTTTTCCg < 2:500046/86‑1 (MQ=255) gcgcGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCAcc > 2:83665/1‑90 (MQ=255) gACGTTTACCGTTTTGGGTCCCAAGCCCCGTTCGGGGGCAATTTTTTTCCCCGCCATTCCTTGCCCCAGTTTTTCCGCCACCTGGCGTTc < 1:40066/90‑1 (MQ=255) gACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTc < 1:83665/90‑1 (MQ=255) gTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGtt > 1:390855/1‑90 (MQ=255) ttCGGTGACAAGCCCAGTTCGGCGGCATTCTCTTCCCCCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGc < 2:267218/90‑1 (MQ=255) tCGGTGACAAGCCCATTTCGGCGGCAATCTCTTTCCCCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCg < 2:33634/90‑1 (MQ=255) ggTGACAGGCCCGGTTGGGCGCCAATCTCTTTCCCCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCggg < 1:494857/90‑1 (MQ=255) ggTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCggg < 1:347014/90‑1 (MQ=255) gCCCAGTTCGGGGGTAATCTCTTTCCCCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTgacg < 2:385320/90‑1 (MQ=255) tctcTTTCCCCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTaa < 1:561180/90‑1 (MQ=255) | GTTACTGACGCCCAGTTTTTCCATCAGATTGGCGCGATGGACGTGTACCGTTTTCGGTGACAAGCCCAGTTCGGCGGCAATCTCTTTCACCGCCATTCCTTGCGCCAGTTTTTCCGCCACCTGGCGTTCACGTTTGGTTAGCGGGTCCTGACGACCGGATGCCAGTTTAA > NZ_CP009273/3843535‑3843704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |