| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,102,506 | 0 | T | G | 54.5% | 4.4 / 12.3 | 12 | G88G (GGT→GGG) | yiiR | DUF805 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAAT‑GGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTT > NZ_CP009273/4102437‑4102584 | gttGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCggg < 2:265271/89‑1 (MQ=255)cTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGTGCCGGCTGGG‑GGGGAACT‑GGGGGGTTTTTCCggg > 2:4332/1‑90 (MQ=255)cTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACT‑GGGGGGTTTTTCCggg > 1:66452/1‑90 (MQ=255) tGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGCGGGGAAAAG‑GGGGGTTTTTACCgggg > 2:566323/1‑89 (MQ=255) cATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTc < 2:116013/90‑1 (MQ=255) gcgGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGGTGTTGGC‑GGGGAAAT‑GGGGGGTTTTTCCCGGGGTTTggggaa > 2:322797/1‑86 (MQ=255) ggCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAAT‑GGGCGGTgggg < 1:496369/90‑1 (MQ=255) ggCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGGAACTGGGGGGATTTAACCGGGT‑TCTGGCAAA‑AGGCGGTgggg > 1:563310/1‑90 (MQ=255) aTTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAAT‑GGGCGGTGGGGCGTTTTGTccc < 2:189527/90‑1 (MQ=255) tttCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAAT‑GGGCGGTGGGGCGTTTTGTCCCg < 1:523867/90‑1 (MQ=255) ttGTGGCGTGGATGCTGCTGGC‑GGGGAAAT‑AGGGGGTTTTGCCCGGGGGTTGGCTATGGGGGGGGGGGGGGTTTTTTCCCGCAGTTGttt > 1:323168/1‑90 (MQ=255) ctgctgGC‑GGGGAAAT‑GGGCGATTTTACCGGGGGTCTGGCAAT‑GGGCGGGGGGGGGGTTTTTCCCGACGTTGATTTTTGGGATGATGCtt > 2:506769/1‑90 (MQ=255) | CTTGCATGATCGCGGGCGTTCCGGCGCATGGGCATTTCTGATGATTGTGGCGTGGATGCTGCTGGC‑GGGTAACT‑GGGCGATTTTACCGGGTGTCTGGCAAT‑GGGCGGTGGGGCGTTTTGTCCCGACGTTGATTCTGGTGATGATGCTT > NZ_CP009273/4102437‑4102584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |