Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273123,3560TG54.5% 7.5 / 10.7 11V392G (GTG→GGG) aceFpyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base G (6/0);  total (9/2)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGG  >  NZ_CP009273/123272‑123441
                                                                                    |                                                                                     
aCGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGGGGGATCCCTGGGATGCTGCCGGGGCCGAAGGGGGGCt                                                                                  >  2:296805/1‑90 (MQ=255)
           gCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGGGGACTTCAGCAAGgtt                                                                       >  2:225526/1‑90 (MQ=255)
            cTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCAAGCTGCCGTGGCCGAAGGGGGAATTCAGCAAGTTTg                                                                      >  1:482937/1‑90 (MQ=255)
                   aCGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGGATCCCTGGCATGCTGCCGGGGCCGAAGGGGGACTTCAGCAAGGTTGGGGAAAt                                                               >  1:392557/1‑90 (MQ=255)
                                gcTCCGGCAGCGACTGGCGGGGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTggga                                                  >  1:590613/1‑88 (MQ=255)
                                    cGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGGGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGTGGAACTgg                                              >  1:524352/1‑90 (MQ=255)
                                       cAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGcc                                           <  2:241262/90‑1 (MQ=255)
                                               ggCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGGGGACTTCAGCAAGTTTGGGGAAATCGAAGAAGGGGAACTGGGGCGCATCCAg                                   >  2:177795/1‑90 (MQ=255)
                                                                   gctgcCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCTGCATCCAGAAAATCTCTGGTGCGAACCt               >  1:249192/1‑90 (MQ=255)
                                                                        cGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGcc          <  1:112794/90‑1 (MQ=255)
                                                                                aaGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTgg  >  1:396358/1‑90 (MQ=255)
                                                                                    |                                                                                     
ACGTGAAAGAAGCTATCAAACGTGCAGAAGCAGCTCCGGCAGCGACTGGCGGTGGTATCCCTGGCATGCTGCCGTGGCCGAAGGTGGACTTCAGCAAGTTTGGTGAAATCGAAGAAGTGGAACTGGGCCGCATCCAGAAAATCTCTGGTGCGAACCTGAGCCGTAACTGG  >  NZ_CP009273/123272‑123441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑