Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273684,9700AC54.5% 6.4 / 12.0 11G456G (GGT→GGG) lntapolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTGAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCGCGC  >  NZ_CP009273/684896‑685053
                                                                          |                                                                                   
gtgAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATc                                                                      >  2:114177/1‑90 (MQ=255)
         gtggtCGGCGTCACGTTAGTGGTTAACCCCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCCCCCTGCGGGCCAATCACCGCCGTa                                                             <  2:76125/90‑1 (MQ=255)
                         tggTGGTAACCCCCCCGGGGGTGAACGGGGGGATCACCGCCTGAATCCCCCCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGtgc                                             <  1:205151/88‑1 (MQ=255)
                         tAGTGGTTACCCCCCCGGGGGTGACCGGGGGGATCACCGCCTGAATCCCCCCCTGGGGGCCAATCCCCGCCGTAATGCCGTTGTTGGtgc                                             <  1:208968/90‑1 (MQ=255)
                             ggTTAACCCCTCGGGGGTGAACTGCGGGATCATCGCCTGAATCTCCCCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTgcg                                         <  2:412857/90‑1 (MQ=255)
                              gTTAACCCCTCGCGGGTGAACTGGGGGATCATCGCCTGAATCTCCCCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTgcgc                                        <  2:176622/90‑1 (MQ=255)
                                    acCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGt                                  >  1:341601/1‑90 (MQ=255)
                                       tCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTggg                               >  2:131260/1‑90 (MQ=255)
                                               gAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAg                       >  1:83516/1‑90 (MQ=255)
                                                           catcGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGcgcacg           >  1:92386/1‑90 (MQ=255)
                                                                    aaTCCCCCCCTGGGGCCCACCCCCCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCgcgc  <  1:61935/90‑1 (MQ=255)
                                                                          |                                                                                   
GTGAGTCCGGTGGTCGGCGTCACGTTAGTGGTTAACACCTCGCGGGTGAACTGCGGGATCATCGCCTGAATCTCACCCTGCGGGCCAATCACCGCCGTAATGCCGTTGTTGGTGCTGCGCAACAGTGGGCGCGCCAGCTCCAGCGCACGCATTCGCGC  >  NZ_CP009273/684896‑685053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑