| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,745 | 0 | A | G | 53.8% | 6.1 / 11.4 | 14 | G171G (GGA→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (7/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTAC > NZ_CP009273/1152657‑1152833 | tGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGGTTTC‑GGAt > 2:370184/1‑90 (MQ=255) aagaaAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATGGGTCTC‑GGGtttttta > 2:277429/1‑87 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGATCTC‑GGGTTTTATATTTTCTTTTCCCTCCTTGTGACCt > 1:313437/1‑90 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGGACTC‑GGGTTTTATATCTCCTTTTCTGGCATTGTGACCTTcccgta > 2:116268/1‑86 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAAATACTTGGTCTCGGGTTTTTTAATTTCTTTTTCCGCGATT‑TGGCCCTCCGTGATACGGAgg > 2:243861/1‑89 (MQ=255) gagGACAGAGAAACGGCGGGGAAAATAATTGATCTC‑GGGTTTTTCATCTCCCTTTTCCGCATTTTGACCTTCCCTAATGCGGGGCaaacg > 1:93156/1‑87 (MQ=255) ggACAGAGAAACGGCGGGGAAATTAATGGATCTC‑GGGTTTTTCATCTCCTTTTTCGGCATTTTGACCTTCCGTAATGCGGGGGAACTgcg > 2:98759/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTTGGTCTC‑GGGTTTTTCATTTCCTTTTTCCGCCTTTTGGCCTTCCGTAATTCGGGGGAACTgcg > 1:181812/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGATCTC‑GGGTTTTACATCTCCTTTTCCCGCATTTTGACCTTCCCTAATTCGGGGCAACcgcg > 2:266888/1‑90 (MQ=255) gACAGAGAAACGGCGGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc < 2:180110/90‑1 (MQ=255) gACAGAGAAACGGCGGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc < 2:93156/90‑1 (MQ=255) tAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 2:182577/90‑1 (MQ=255) cTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTg > 2:384097/1‑90 (MQ=255) gATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTAc < 1:266888/90‑1 (MQ=255) | TGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTC‑GGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGTCCCCCTGGATCGGTTAC > NZ_CP009273/1152657‑1152833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |