| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,994,523 | 0 | A | C | 56.2% | 6.7 / 20.2 | 16 | T287P (ACC→CCC) | xdhA | xanthine dehydrogenase molybdenum‑binding subunit XdhA |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base C (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTC > NZ_CP009273/2994440‑2994607 | agCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTa > 2:374098/1‑90 (MQ=255) ttCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCtttt > 1:446448/1‑90 (MQ=255) gCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACg > 1:189536/1‑90 (MQ=255) gCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACg > 2:438274/1‑90 (MQ=255) tGGCGGCTTCCGGGTGAATTTTCCCC‑TTAGCGGTGAGGGGTTTTTCCCCGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAAt < 1:132894/90‑1 (MQ=255) tGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAAt > 2:205220/1‑90 (MQ=255) tGGCGCCTTTCCGGTGAATTTTCCCC‑TTAGCCGTGAAGGGTTTTCCCCCGCACCCCCTCCCCGCCACGCTTTTACCATTGACGGGCAAAt < 1:438274/90‑1 (MQ=255) gggcggcATTCGGGAAAACTTTCCCC‑TAAGGGGTGAAGTTTTTTCCCCCCCACCCCCTCCCCGCCACGCTTTTACCATTGACGGGCAAAt < 1:197960/89‑1 (MQ=255) gggcggcATGCGGGAAAATGTTTCCC‑TTGGCGGTGAGGGGTTTTCCCTCGCACCCCTCCCCCGCCACGCTTTTACCATTGACGGGCAAAt < 1:415370/89‑1 (MQ=255) gggcggc‑TTCCGGGAAAAATTTCCCTTTAGCGGGGAAGGGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAAt < 1:3629/89‑1 (MQ=255) ggTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcg > 2:5533/1‑90 (MQ=255) ggAAAATTTCCCTTTCCCCGTGAAGAGTTTTCCCCCCCCCCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACcgc < 2:446448/89‑1 (MQ=255) gAAGAGTTTTCCCCCCCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTtat < 2:18508/90‑1 (MQ=255) ttcccTCCCACCCCTTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGa < 2:246049/87‑1 (MQ=255) tCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTc > 1:428209/1‑90 (MQ=255) ccGCACCCCGTCCCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTc < 1:355097/89‑1 (MQ=255) | AGCCAATGGCGGCATTCCTGACCAGCAAGCTTGGCGGCATTCCGGTGAAAGTTTCCC‑TTAGCCGTGAAGAGTGTTTCCTCGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTC > NZ_CP009273/2994440‑2994607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |