| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,964,457 | 0 | T | G | 54.5% | 6.3 / 13.0 | 11 | V322G (GTG→GGG) | wecB | UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (6/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964368‑3964545 | cATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGt > 1:128724/1‑90 (MQ=255) aGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTc < 2:405022/90‑1 (MQ=255) agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGt < 2:128724/90‑1 (MQ=255) agaagCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGGGGCTggg > 2:403138/1‑89 (MQ=255) agCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGTAgg > 2:147075/1‑90 (MQ=255) gtgCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGGGGGCCGGAAAAACAGCGAATTGTc > 1:115562/1‑90 (MQ=255) gATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGGAGGGGGCGTCTGGTAGGGATGGATAAACAGCGAATTTTCggggggtt > 1:35921/1‑86 (MQ=255) gAGCGTCCGGAAGCGGTGACGGCGGGTACGGGGCGTCTGGTAGGGACGGGTAAGCAGCGAATTTTCGAGGGAGGGACGCGGCTTTTtaaa > 2:147811/1‑90 (MQ=255) gTCCGGAAGCGGTGACGGCGGGGACGGGGCGGCTGGGAGGGACGGAAAAACAGCGAAATGTTGGGGGAGGGGCGCGGCTTTTtaaaaaag > 1:211997/1‑86 (MQ=255) gAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAAc < 2:37027/90‑1 (MQ=255) aaGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACg < 2:211997/90‑1 (MQ=255) gTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCcgc < 2:115562/90‑1 (MQ=255) | CATTCAGGAAGAAGCGCCTTCGCTGGGGAAACCTGTGCTGGTGATGCGCGATACCACTGAGCGTCCGGAAGCGGTGACGGCGGGTACGGTGCGTCTGGTAGGCACGGATAAGCAGCGAATTGTCGAGGAAGTGACGCGTCTTTTAAAAGACGAAAACGAATATCAAGCTATGAGCCGC > NZ_CP009273/3964368‑3964545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |