Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,102,3560TG53.8% 7.3 / 12.9 13G38G (GGT→GGG) yiiRDUF805 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base G (7/0);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAG  >  NZ_CP009273/4102280‑4102434
                                                                            |                                                                              
gTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCg                                                                   <  2:374048/90‑1 (MQ=255)
          ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAACTACCCGATATTCAGAc                                                         >  1:117/1‑90 (MQ=255)
          ccGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAAAAATTACTCGATATTTAGAc                                                         >  1:132282/1‑90 (MQ=255)
                      gATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTg                                             <  1:102670/90‑1 (MQ=255)
                                ggCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAAAATACTCCATATTTAAACCCCGGCGGTTTTCCTtttgtgg                                   >  1:385231/1‑89 (MQ=255)
                                ggCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAAAATCTACTCGATATTTAGACCCCGGCGGTTTTCCTTTTGtgc                                   >  2:351894/1‑90 (MQ=255)
                                     gtgGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGGAAGAATCTACTCGATATTTAGACCGCGGCGTTTTGCCTTGTGtggttgct                              >  1:40901/1‑90 (MQ=255)
                                                gCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAg                   <  1:32152/90‑1 (MQ=255)
                                                  aTGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCg                 <  1:192391/90‑1 (MQ=255)
                                                  aTGCTGGTGCTTTTCTCACTGGCGGGGAAGAATCTACTCGATATTCAGACCGCGGGGGTTTTCCTTGTGTGCTTGCTCTCGCCCACAGCg                 >  1:347108/1‑90 (MQ=255)
                                                  aTGCTGGTGCTTTTCTCACTGGCGGGGAAGAAATTACTCCATATTTAGACCGCGGCGGTTTTCCTTTTGTGCTTGTTCTGGCCCACAACg                 >  1:294704/1‑90 (MQ=255)
                                                             tttCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCCCGGCGGTTTTCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGGAACTGt      >  2:47578/1‑90 (MQ=255)
                                                                 tcACTGGCGGGTAAGAATCTACTCGGTATTTAGACCGCGGCGTTTTGCCTTGTGGGGTTGCTCTGGCCAACAGCGGCCGTAACTGtttaa  >  1:45200/1‑87 (MQ=255)
                                                                            |                                                                              
GTATTGGACGCCGTGATTTCTGGATTTGGATAGGCCTGTGGTTCGCAGGCATGCTGGTGCTTTTCTCACTGGCGGGTAAGAATCTACTCGATATTCAGACCGCGGCGTTTTGCCTTGTGTGCTTGCTCTGGCCAACAGCGGCAGTAACTGTTAAG  >  NZ_CP009273/4102280‑4102434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑