Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	214,743	A→C	Y174D (TAC→GAC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	214,743	0	A	C	100.0%	67.1 / NA	18	Y174D (TAC→GAC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/6);  total (12/6)

GGTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTAGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGA  >  NZ_CP009273/214666‑214823                                                                              |                                                                                 ggTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAg                                                                      >  2:136218/1‑90 (MQ=255)      cGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGAtt                                                                 >  1:63869/1‑90 (MQ=255)            gTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTgag                                                           <  1:182898/90‑1 (MQ=255)                ggCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGt                                                       <  1:240027/90‑1 (MQ=255)                 gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAggg                                                                    >  1:258192/1‑76 (MQ=255)                 gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAggg                                                                    <  2:258192/76‑1 (MQ=255)                 gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTa                                                      >  2:364344/1‑90 (MQ=255)                 gcgcGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTa                                                      >  2:132905/1‑90 (MQ=255)                  cgcgGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTAt                                                     >  2:308250/1‑90 (MQ=255)                      gAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGaa                                                 <  2:9587/90‑1 (MQ=255)                      gAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGaa                                                 <  2:170977/90‑1 (MQ=255)                                   aTGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCt                                    >  2:145807/1‑90 (MQ=255)                                                  ttGCTGTAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTcg                     >  2:422393/1‑90 (MQ=255)                                                        tAGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAATTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGaa               <  1:53650/90‑1 (MQ=255)                                                         aGGCCGCATACATTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAAc              >  2:30901/1‑90 (MQ=255)                                                                    aTTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCg   >  2:20456/1‑90 (MQ=255)                                                                    aTTGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCg   >  2:425696/1‑90 (MQ=255)                                                                     ttGCATCGTCGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGa  >  1:73051/1‑90 (MQ=255)                                                                              |                                                                                 GGTGTCGGCTTGTACGGCGCGGAAATCCAGCCCCATGCGGCTGAAGATTTTGCTGTAGGCCGCATACATTGCATCGTAGGTTTCCTGCAGGGATTCCTGAGAAGTATGGAAAGAGTAAGCATCTTTCATCAGGAATTCGCGGGAACGCATGACGCCGA  >  NZ_CP009273/214666‑214823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑