| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 353,601 | 0 | C | A | 58.8% | ‑3.4 / 15.4 | 17 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
| Reads supporting (aligned to +/- strand): ref base C (5/2); new base A (0/10); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC > NZ_CP009273/353525‑353685 | cacccGCGTTTTTACCCACCCCCATGTGAAAAAA‑TTCCTTCGGGGGGGCTGTCTCCCAGTTTTTTTC‑CCGCCGCAACAAAACCGCCGTtc < 2:508055/87‑1 (MQ=255)cacccGCGCATTTATCCACCGCCATGTGAAAAAA‑TGCCTTCGCGGGGGCTGTCTGCCAGCTTTTTTC‑CCGCCGCAACAAAACCGCCGTtc < 2:74454/87‑1 (MQ=21) tttATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCagtag > 1:55577/1‑90 (MQ=255) cccACCGCCATG‑GCAAAAAGTGCCTCCGGGGGGGTTTTCTGCAAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc < 2:297281/89‑1 (MQ=255) aCGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:86319/1‑90 (MQ=255) aCGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:39726/1‑90 (MQ=255) aCGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:309859/1‑90 (MQ=255) gCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGGAACAAAACCGCCGTTCTCTCCAGTAGTGGCggacct > 2:312529/1‑86 (MQ=255) aaaaaa‑TGCCTCCGGGGCGGCTTTCTGCCAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 2:315285/89‑1 (MQ=255) aaaaaa‑GCCCTTCGGGGCGGTTGTCGCCCAGTTTTTTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAAt < 2:45734/89‑1 (MQ=255) ttccgcgGGGGTTGTCTCCCAGTTTTTTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAg < 2:423353/87‑1 (MQ=255) tCGGGGCGGTTGTCGGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGcc < 2:137814/90‑1 (MQ=255) cgggggcggcTGTCGGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTCAAGcc < 2:136919/86‑1 (MQ=255) gcTGTCGGCC‑GCTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCAtgt < 2:13135/90‑1 (MQ=255) tttttc‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:233345/89‑1 (MQ=255) tttttc‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgc < 2:150005/89‑1 (MQ=255) ttccccgccaAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctg < 1:499683/86‑1 (MQ=255) cgaaacaaAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 2:357620/87‑1 (MQ=255) | CAACCGCGCATTTATCCAACGCCATGTGCAAAAA‑TGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAC > NZ_CP009273/353525‑353685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |