| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,026 | 0 | T | G | 57.1% | 9.6 / 13.9 | 15 | G209G (GGT→GGG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGA > NZ_CP009273/699944‑700105 | gTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGgtt > 1:405996/1‑90 (MQ=255) tCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCCCGGGGACATTAACCGCTTCTa > 2:139815/1‑90 (MQ=255) tGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTg < 1:139815/90‑1 (MQ=255) tGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGCACCGTTTTCCCCGGGGGAATTTAACGCTTCTCTGcccgcg > 1:212074/1‑86 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 1:45525/90‑1 (MQ=255) acCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGc < 1:528119/90‑1 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCCCGGGGACATTAACCGCTTCTTTGCCGGTGACGGCa > 2:92044/1‑90 (MQ=255) ccATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGGGGCATTAAACGCCTTTTTCCCGGGGACGGCa > 1:269025/1‑90 (MQ=255) aTCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCACGGGGACATTAACCGCTTCTATGCCCGCGACGGCAcc > 2:150677/1‑90 (MQ=38) cGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACcgc < 1:537067/90‑1 (MQ=255) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGTTTTCCACGGGGACATTAACCGCTTCTATGCCGGGGACGGCACcgcg > 1:137868/1‑90 (MQ=39) gCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGGACGGGTTTTCACGGTGACATTAACCGCTTCTATGCCGGGGGCGGGACcgcg > 2:405799/1‑90 (MQ=255) ggTGAATTCACCAACGCGGCGGGTACGGGTTTCCACGGGGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTcc > 2:33532/1‑90 (MQ=255) aTTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGctt < 2:254613/90‑1 (MQ=255) aCGCGGCGGGGACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGGTGTTCATGTCCGGCTTCTTCCCGa > 2:99227/1‑90 (MQ=255) | GTCTGCTGATCCCAACCGGTCTGCATCAGGTACTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGTACGGTTTTCCACGGTGACATTAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGA > NZ_CP009273/699944‑700105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |