| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,872,539 | 0 | G | T | 53.3% | 11.2 / 22.4 | 15 | E190* (GAA→TAA) | dgcP | diguanylate cyclase DgcP |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAT > NZ_CP009273/1872454‑1872623 | aCAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTaaaa > 1:518219/1‑90 (MQ=255) aaGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGacac < 1:415189/90‑1 (MQ=255) aaGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGacac < 1:66790/90‑1 (MQ=255) tgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATCTGACGACACTGTTTTtccc > 1:54978/1‑89 (MQ=255) gcgcTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGGTTTTTTAAAATCTGACGACACTGTTTTCCCTc > 1:27335/1‑90 (MQ=255) gATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATTTTACGACACTGTTTTCCCTCCCCCg > 2:499802/1‑90 (MQ=255) cAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGACGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCtt < 2:513038/90‑1 (MQ=255) tataCCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCAt < 2:525935/90‑1 (MQ=255) gACTCGTTAACCGGGCTACCGAATCGGCGGGGGGTTTTTTAAAAATTGAAGAAAATGTTTTTCCGCGCCCGGCATACTTACCATaaagtt > 2:347371/1‑86 (MQ=255) gACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATCTGACGACACTGTTTTTCCTCGCCCCGCATCTTTACCATAAAATa > 2:455050/1‑90 (MQ=255) gACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTTAAAATCTGACGAAACTGGTTTCCCTCGCCCGGCAGCTTAACCATAAAATa > 1:325069/1‑90 (MQ=255) gACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATa > 2:228110/1‑90 (MQ=255) gCTACCGAATCGGCGGGCGATTTTTTAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTAt > 1:393835/1‑90 (MQ=255) cTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATAGCGTTTATc < 1:499802/90‑1 (MQ=255) tttttGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAt < 1:228110/90‑1 (MQ=255) | ACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAT > NZ_CP009273/1872454‑1872623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |