| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,738,448 | 0 | A | C | 53.3% | 5.9 / 16.2 | 15 | V84G (GTG→GGG) | trmD | tRNA (guanosine(37)‑N1)‑methyltransferase TrmD |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base C (0/8); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.15e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG > NZ_CP009273/2738359‑2738537 | gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa > 1:190314/1‑90 (MQ=255)gACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa > 2:490123/1‑90 (MQ=255) ccAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTgcgc < 2:84936/90‑1 (MQ=255) tCAATTTTTGTTTCGTTGCCCGTTCGCTGACGCCCGCTTGATCAAGCTTGCGCCCCTGTGGTGACAGAAAAATCCCCTTTGCGCCTTccc < 1:312403/90‑3 (MQ=255) cAATTTTTGATTCGTTGCCAGTTCGCTGACCCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTcccc < 1:445601/90‑1 (MQ=255) ccAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTtgctg < 2:526458/90‑1 (MQ=255) gACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGc < 2:190314/90‑1 (MQ=255) tCAAGCTTGCGTCCTTGTGGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGt < 1:228076/90‑1 (MQ=255) cTTGCGTCCGTTTGGTGATAAATAAATCCCCTTTGCTCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 2:416002/90‑1 (MQ=255) cTTGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCAc < 2:8714/90‑1 (MQ=255) tGCGTCCCTGTGGTGACAGATAAATCCCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACca < 2:194738/90‑1 (MQ=255) tgtgGTGACAGATAAATCCCCTTTGCGCCTTCCCCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAAc < 1:104120/90‑1 (MQ=255) ggtgACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATc > 1:54170/1‑90 (MQ=255) gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATcccc > 1:224302/1‑90 (MQ=255) gACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGGTGCACCATCATTAACATcccc > 2:78402/1‑90 (MQ=255) gATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTc > 1:211830/1‑90 (MQ=255) aTAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTcc > 1:114550/1‑90 (MQ=255) aCCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTccgccgccg > 2:182716/1‑90 (MQ=255) | GACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTTTGCGCCTTCACCCGCCGCGGCTTTTGCTGCATGAATGGCGTCCCGCAAGGGTTGCACCATCATTAACATCCCCGGTCCGCCGCCG > NZ_CP009273/2738359‑2738537 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |