| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,912 | G→T | W30C (TGG→TGT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,912 | 0 | G | T | 96.3% | 89.4 / ‑2.7 | 27 | W30C (TGG→TGT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (7/19); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA > NZ_CP009273/49828‑49996 | cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa > 2:274678/1‑90 (MQ=255)cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaa > 1:496195/1‑90 (MQ=255) aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTaaa > 1:500575/1‑90 (MQ=255) tCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACg > 1:451250/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCTGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAAGCaa > 1:417227/1‑90 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 2:500575/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 2:443143/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 2:462180/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 1:32453/90‑1 (MQ=255) gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCaa < 1:284256/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt < 2:122057/90‑1 (MQ=255) cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCt < 1:293272/90‑1 (MQ=255) ttAGCGGTAGATCGCGTTATCGGCATGGAAAACCCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaat < 2:451250/90‑1 (MQ=255) aggggTAGATCGCGTTATCGGCATGAAAAACCCCAGGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaataa < 1:403695/87‑1 (MQ=255) gCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTtaaataaa < 2:50113/90‑1 (MQ=255) aGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCGGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGt < 2:498739/90‑1 (MQ=255) ttATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg < 2:522008/90‑1 (MQ=255) tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg < 2:438718/90‑1 (MQ=255) ggaaTGGAAACCGCCATGCCGTGGACCCTCCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgc < 1:274678/87‑1 (MQ=255) aTGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt < 2:157548/90‑1 (MQ=255) cGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa < 2:298666/90‑1 (MQ=255) gCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaa < 2:115728/90‑1 (MQ=255) aTGCCGTGGAACCTGCCTGCCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca > 2:208805/1‑90 (MQ=255) ctgcctgcCGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg > 1:384766/1‑90 (MQ=255) tgcctgccGATCTCGCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt > 2:239229/1‑90 (MQ=255) ctcccGCCTGTTTTAAACGCAACACCTTAAATAACCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGAc < 2:384766/86‑1 (MQ=255) gCCTGTTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCaaa < 1:494422/90‑1 (MQ=255) | CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAA > NZ_CP009273/49828‑49996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |