New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 19727510 (0.000)82 (1.520) 46/168 0.0 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP

Only 100 of 110 total aligned reads displayed.

ACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGG  >  NZ_CP009273/1972664‑1972840
                                                                                       |                                                                                         
acgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACa                                                                                         <  2:181451‑M2/7‑1 (MQ=255)
aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAgg                                                                                         >  1:860515‑M1/1‑88 (MQ=255)
 cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTTAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAgga                                                                                        <  1:516093‑M1/90‑4 (MQ=255)
  tCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaa                                                                                       <  1:777034‑M1/90‑5 (MQ=255)
  gggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATa                                                                                       <  2:448884‑M2/9‑1 (MQ=255)
   cGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaag                                                                                      >  1:458892‑M1/1‑85 (MQ=255)
   cGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaag                                                                                      >  2:385945‑M1/1‑85 (MQ=255)
    gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATaaa                                                                                     <  2:1014274‑M2/11‑1 (MQ=255)
    gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATaaa                                                                                     <  2:1182399‑M2/11‑1 (MQ=255)
    gagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATaaa                                                                                     >  1:1331670‑M2/80‑90 (MQ=255)
     tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAtgaaggt                                                                                    >  1:113825‑M1/1‑83 (MQ=255)
     tCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggt                                                                                    >  1:61583‑M1/1‑83 (MQ=255)
        cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcg                                                                                 >  1:274230‑M1/1‑80 (MQ=255)
         aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGtat                                                                                <  1:652630‑M2/16‑1 (MQ=255)
         aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGtat                                                                                <  1:619360‑M2/16‑1 (MQ=255)
         aaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGtat                                                                                <  1:1177609‑M2/16‑1 (MQ=255)
             cGCACATGCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAGCACTCAATTACTTAAggaaggtgcgaacaa                                                                            >  1:129468‑M1/1‑75 (MQ=255)
             cGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaa                                                                            <  1:1028235‑M1/90‑16 (MQ=255)
               ggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCa                                                                          >  2:1151237‑M2/69‑90 (MQ=255)
               cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagt                                                                          >  2:1042274‑M1/1‑73 (MQ=255)
               cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagt                                                                          >  1:825227‑M1/1‑73 (MQ=255)
               cacaTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagt                                                                          >  2:989757‑M1/1‑73 (MQ=255)
                gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCAt                                                                         <  1:924280‑M2/23‑1 (MQ=255)
                gctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCAt                                                                         <  2:259866‑M2/23‑1 (MQ=255)
                  aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccc                                                                       <  1:385945‑M1/90‑21 (MQ=255)
                  aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccc                                                                       >  2:1125465‑M1/1‑70 (MQ=255)
                  aTACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccc                                                                       >  1:1472040‑M1/1‑70 (MQ=255)
                    aCAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctg                                                                     <  1:1251560‑M1/90‑23 (MQ=255)
                       aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgata                                                                  >  1:502200‑M1/1‑65 (MQ=255)
                        cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatat                                                                 <  1:832415‑M1/90‑27 (MQ=255)
                           aGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgag                                                              >  1:330813‑M1/1‑61 (MQ=255)
                            ggggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccc                                                                       >  1:397181‑M1/1‑60 (MQ=255)
                            ggggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccc                                                                       <  2:397181‑M1/80‑21 (MQ=255)
                            agaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACttttgttttg                                                             <  1:347547‑M2/35‑1 (MQ=255)
                            agaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACttttgttttg                                                             <  2:789917‑M2/35‑1 (MQ=255)
                             gggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagat                                                            >  2:1463696‑M1/1‑59 (MQ=255)
                              ggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatc                                                           >  2:189414‑M1/1‑58 (MQ=255)
                               gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatca                                                          >  1:860174‑M1/1‑57 (MQ=255)
                                atgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGgtg                                                         <  2:587609‑M2/39‑1 (MQ=255)
                                atgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGgtg                                                         <  1:1155664‑M2/39‑1 (MQ=255)
                                atgaaatgactgagtcagcagagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGgtg                                                         <  1:1021404‑M2/39‑1 (MQ=255)
                                 gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatg                                                        >  2:563266‑M1/1‑55 (MQ=255)
                                    gCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgttt                                                     >  2:248449‑M1/1‑52 (MQ=255)
                                      gATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgt                                                   >  1:820144‑M1/1‑50 (MQ=255)
                                       gactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACttt                                                                    >  1:726295‑M2/45‑72 (MQ=255)
                                       gactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACttt                                                                    <  2:726295‑M2/28‑1 (MQ=255)
                                       gactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCa                                                  >  1:1389822‑M2/45‑90 (MQ=255)
                                       gactgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCa                                                  >  2:1320738‑M2/45‑90 (MQ=255)
                                          tgagtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc                                               <  1:939115‑M2/49‑1 (MQ=255)
                                            agtcagccgagaagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTg                                             >  1:777081‑M2/40‑90 (MQ=255)
                                              ataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                           <  2:506553‑M1/90‑49 (MQ=255)
                                              ataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                           <  2:1472040‑M1/90‑49 (MQ=255)
                                                aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagc                                         <  1:630823‑M1/90‑51 (MQ=255)
                                                aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagc                                         <  1:354924‑M1/90‑51 (MQ=255)
                                                 atGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcc                                        >  1:1540886‑M1/1‑39 (MQ=255)
                                                 atGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcc                                        >  1:290410‑M1/1‑39 (MQ=255)
                                                 atGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcc                                        >  2:736885‑M1/1‑39 (MQ=255)
                                                  tGCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcca                                       <  2:211060‑M1/90‑53 (MQ=255)
                                                   gCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      <  2:993349‑M1/90‑54 (MQ=255)
                                                   gCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      <  1:130135‑M1/90‑54 (MQ=255)
                                                   gCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      <  2:1540886‑M1/90‑54 (MQ=255)
                                                   gCGTGATGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      >  2:101894‑M1/1‑37 (MQ=255)
                                                       aagaatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGttt                                  <  2:673056‑M2/62‑1 (MQ=255)
                                                        aTGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaac                                 >  2:330787‑M1/1‑32 (MQ=255)
                                                        aTGCAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaac                                 <  1:736885‑M1/90‑59 (MQ=255)
                                                          aatttccccgcttattcgcaccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTc                               <  2:628389‑M2/65‑1 (MQ=255)
                                                           cAGATCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagg                              <  1:921406‑M1/90‑62 (MQ=255)
                                                              aTCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggtt                           <  1:1259612‑M1/90‑65 (MQ=255)
                                                               tCACACAAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttc                          >  1:1343941‑M1/1‑25 (MQ=255)
                                                                  cacaAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatc                       <  1:189414‑M1/90‑69 (MQ=255)
                                                                  cacaAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      >  1:619353‑M1/1‑22 (MQ=255)
                                                                  cacaAAACACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccat                                      <  2:619353‑M1/75‑54 (MQ=255)
                                                                     aaaaCACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatg                    <  2:580029‑M1/90‑72 (MQ=255)
                                                                      aaaCACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                   >  1:1271416‑M1/1‑18 (MQ=255)
                                                                      aaaCACTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                   <  2:416662‑M1/90‑73 (MQ=255)
                                                                         cacTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtc                <  1:243941‑M1/90‑76 (MQ=255)
                                                                          acTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtca               <  2:1413905‑M1/90‑77 (MQ=255)
                                                                           cTCAATTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcat              >  1:963010‑M1/1‑13 (MQ=255)
                                                                              ccttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCaaaaa           >  2:1345848‑M2/6‑90 (MQ=255)
                                                                              aaTTACTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaa           >  2:1000048‑M1/1‑10 (MQ=255)
                                                                                ttcCTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATg         >  1:1397693‑M2/4‑90 (MQ=255)
                                                                                  aCTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaactta       >  2:899490‑M1/1‑6 (MQ=255)
                                                                                   cTTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttac      >  2:1126294‑M1/1‑5 (MQ=255)
                                                                                    ttAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacc     >  2:1015332‑M1/1‑4 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacct    >  1:1230871‑M1/1‑3 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacct    >  2:231285‑M1/1‑3 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacct    >  2:495529‑M1/1‑3 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacct    >  2:1414477‑M1/1‑3 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                           >  2:956371‑M1/1‑3 (MQ=255)
                                                                                     tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                           <  1:956371‑M1/51‑49 (MQ=255)
                                                                                      aaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacctt   >  2:66408‑M1/1‑2 (MQ=255)
                                                                                      aaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctgga                                           >  1:405982‑M1/1‑2 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  >  2:455645‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  >  1:214706‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  <  2:1343941‑M1/90‑90 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  >  1:60552‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  >  2:969679‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttc  >  1:8491‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaaca                                >  1:1268078‑M1/1‑1 (MQ=255)
                                                                                       aggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaaca                                <  2:1268078‑M1/60‑60 (MQ=255)
                                                                                       |                                                                                         
ACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGG  >  NZ_CP009273/1972664‑1972840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.