| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,957 | 0 | A | G | 53.3% | 26.5 / 40.1 | 30 | L494P (CTC→CCC) | ybhF | ATP‑binding cassette domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (1/13); new base G (16/0); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.17e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.56e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA > NZ_CP009273/822870‑823045 | gCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAgg < 1:1340847/90‑1 (MQ=255) cATAAAGTGGGTGGTGACCATCGCCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAAc < 1:1070110/90‑1 (MQ=255) tgggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGaa > 2:1491657/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGTAACGCGGGGAg > 2:1377656/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGAAg > 2:992331/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACACCGGGAg > 2:876385/1‑90 (MQ=255) gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCGAAGCCGGAAg > 2:841835/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtc < 1:876385/90‑1 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGtc > 1:94512/1‑90 (MQ=255) gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGtc > 1:1479653/1‑90 (MQ=255) aCCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGGCGGGtcg > 2:408834/1‑90 (MQ=255) aTCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTtcgtcg < 1:841835/90‑1 (MQ=255) cGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGaaa < 1:695509/90‑1 (MQ=255) gACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACCCCCGGAGGCGGGTCGTCGGGaaaaaa > 1:1100976/1‑87 (MQ=255) ttCTCTACGATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:1476473/90‑1 (MQ=255) ttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTcc < 1:7858/90‑1 (MQ=255) cATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGGGGGCGGGTCGTCGAGAAACAGAAAGTCCGGGGCATg > 1:1223850/1‑90 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACGCCGGCAGTCGGGTCGGCGGGAAAAAGAAAGGCCGGGTCATgg > 1:1209906/1‑89 (MQ=255) aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGc > 2:1118813/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGGGAAAAAGAATGTCCGGGTCTTGCATCAg > 2:957943/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGTCGGGAAAAAGAAAGTCCGGGTTATTCATCAg > 1:198942/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGGTCGGCGGGAAAAAAAAAGTCCGGGTCGTGCATCAg > 1:732871/1‑90 (MQ=255) gTTGATGTGCAGCCAAAATTCACGGCGGGGGAGGGGGGCAACGCCGGAAGTCGGGTCGTCGAGAAACAGAAAGTCCGGGTCATGCATCAg > 1:593853/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGGGGGGGGCAACGCCCGAAGGCGGGTTGTCGGGAAACAAAAAGTCCGGGTCATGCATCAACGGAc > 2:1265928/1‑90 (MQ=255) tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc < 2:1405332/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:1479653/90‑1 (MQ=255) cggcggGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGc < 2:94512/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 2:1209906/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 1:1265928/90‑1 (MQ=255) gAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTaaa < 2:231395/90‑1 (MQ=255) | GCTTCATCCATAAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCCAGCGCCAGCCGCTGTTTAAA > NZ_CP009273/822870‑823045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |