| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 59.1% | 14.3 / 22.0 | 22 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (2/7); new base G (13/0); total (15/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.61e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152649‑1152816 | ggCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTggg > 1:1271077/1‑89 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTAATTGATTTCGGGttt > 2:746672/1‑90 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGGTCTCGGGttt > 2:1415664/1‑90 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATTTCggggtt > 2:530271/1‑86 (MQ=255) aaaaGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACAtctc < 1:342891/90‑1 (MQ=255) gACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATGGGTCTCGGGGTTTATATCTCCttttt > 1:1523567/1‑89 (MQ=255) gATTGCGGTGGCGTACTTCACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGc < 1:423237/90‑1 (MQ=255) ggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGAcc < 1:267856/90‑1 (MQ=255) ggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGAcc < 2:1363919/90‑1 (MQ=255) ggCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTAAATCTCCCTTTTCGGCATTGTGGcc > 2:539837/1‑90 (MQ=255) gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTc < 1:941944/90‑1 (MQ=255) ctacACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTAAATCTCCTTTTTCGGCATTGTGACCTTCCGt > 2:535585/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGTAAATAAAATGATCTCCGGTTTTAAATTTCCTTTTTCCGCATTGGGAGCATCCGTCAAGCgc > 1:1222059/1‑89 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTTGATCTCTGGTTTTATATCTCCTTTTTCCGCCCTGTTACCTTTCCTAATTCgg > 2:1255671/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTTGATCTCGGGTTTTAAATCTCCTTTTTCGGCATTGTGGCCTTCCCTAATGCgg > 2:253471/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATCTCGGGTTTTTTATCTCCCTTTTCTGCATTGTGGACTTCCCTAATGCgg > 1:1490200/1‑90 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 1:112509/90‑1 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 1:10737/90‑1 (MQ=255) ggACAGAGAAACGGCGGGTAAATTACTTGATCTCGGGTTTTACATCTCCTTTTTCCGCATTGTGGGCTTCCCTTATTCGGGGCAAATgcg > 2:1084765/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTTCATTTCCCTTTTCTGCATTGGGAACCTCCCCAATGCGGAGCAACcgcg > 1:681107/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATTGATTTTCGGTTTTATATCTCCCTTTTTCGCATTGGGAACCTTCGCAATGCGGGGCAAATgcg > 1:922124/1‑90 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTATATCTCCCTTTTCCCCATTGTGAACTTCCCTAATGCGGAGCAACTgcg > 1:1340571/1‑90 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:406361/90‑1 (MQ=255) | GGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152649‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |