Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,023,5910TG52.9% 9.2 / 12.3 17V191G (GTG→GGG) yedAdrug/metabolite exporter YedA
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base G (9/0);  total (14/3)
Fisher's exact test for biased strand distribution p-value = 8.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGCATTACCTTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGC  >  NZ_CP009273/2023528‑2023652
                                                               |                                                             
cgcgCATTACCTTACCTGTAGGGATGATGGCGGGGGGGGTTGAGGTGCTGGGGGCAGGGGGGGGGTTTATGATCGCGGCGGTGGTTgggg                                     >  2:537478/1‑90 (MQ=255)
            tacctGTAGGGATGATGGCGGGGGGGATTTGGATGGGGGCGGCAGGGGGGGTGGTGATTTTCTGGTCGGTGTGTTCGGGGGAAAAAcggg                         >  1:721776/1‑87 (MQ=255)
            tacctGTAGGGATGATGGCGGGGGCGATTGAGATGCTGGGGGGAGGCGGGGGGGTAATGATCGCGTCGGTGATTGGGGGGGGAAAAATgg                         >  1:720573/1‑89 (MQ=255)
                tGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGgc                     <  1:604252/90‑1 (MQ=255)
                  tAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGgcgc                   >  2:1326485/1‑90 (MQ=255)
                  tAGGGATGATGGCGGGGGCGATTGAGATGCTGGCGGCAGGCGCGGGGTTAATGATGGCCTCGGTGATTGCGGGGGGAAAACCGACCgcgc                   >  1:1194536/1‑90 (MQ=255)
                   aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGGGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCt                  >  2:315472/1‑90 (MQ=255)
                   aGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGGGGGGTTAATGATCGCGTCGGTGGGTGGGGGGGAAAAACTGACGGCGCt                  >  1:120348/1‑90 (MQ=255)
                      gatgatGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGGGTTAATGATCGCGTCGATGATTGCGGGGGAAAAACTGACGGCGCtccc               >  2:518902/1‑90 (MQ=255)
                        tgatgGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCtccctt             >  2:154693/1‑90 (MQ=255)
                        tgatgGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGGGAAAAACTGACGGCGCtccctt             >  2:643932/1‑90 (MQ=255)
                        tgatgGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGGGAAAAACTGACGGCGCtccctt             >  2:1433244/1‑90 (MQ=255)
                         gatgGCGGGGGCGGTTGGGATGCTGGCGGCAGGGGGGGGGGTAATGATCGGGGCGGTGATTTCGGGGGAAAAACTGAAGGGGCtcccttc            >  1:1277495/1‑90 (MQ=255)
                           tgGCGGGGGCGATTGAGATGCTGGCGGCAGGGGGGGGGGTAATGATCGCGGCGGTGATTGGGGGGGGAAAAATGGCGGGGCtcccttccc          >  1:548706/1‑90 (MQ=255)
                               gggTGCGATTGAGATGCTGGCGGCAGGCGTGGGGTTAATGATCGCGTCGATGATTGCGGGGGAAAAAATGACGGCGCTCCCTTCCCTTTc      >  1:630596/1‑90 (MQ=255)
                                   gCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGc  <  2:1032082/90‑1 (MQ=255)
                                   gCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGc  <  2:620101/90‑1 (MQ=255)
                                                               |                                                             
CGCGCATTACCTTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGC  >  NZ_CP009273/2023528‑2023652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑