Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,370,7550AC54.5% 6.6 / 12.4 11V106G (GTT→GGT) menH2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATG  >  NZ_CP009273/2370687‑2370844
                                                                    |                                                                                         
gATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGcc                                                                      >  1:1386833/1‑90 (MQ=255)
       aCGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCa                                                               <  1:1489017/90‑1 (MQ=255)
             aCGTTCCGCACGTTGTTCACCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAgccgc                                                         <  1:432483/90‑1 (MQ=255)
                tcccGCCCGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCcat                                                      <  1:1414408/88‑1 (MQ=255)
                       cGTTTTTCAGCTTTTTGCAGCCCGGGATGCCCCCCTCCGCCAATACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGcc                                               <  1:1154856/90‑1 (MQ=255)
                          tgttCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCAcc                                            >  2:975876/1‑90 (MQ=255)
                                     ttGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAg                                 >  2:1052724/1‑90 (MQ=255)
                                          gCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGt                            >  2:350250/1‑90 (MQ=255)
                                                         cTTCGACAATACCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa             <  1:1052724/90‑1 (MQ=255)
                                                         cTTCGAAAAAACCCCCACAAACCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGa             <  1:1580391/90‑1 (MQ=255)
                                                          ttCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGaa            >  1:1155761/1‑90 (MQ=255)
                                                                    aCCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGAtg  >  2:348467/1‑90 (MQ=255)
                                                                    |                                                                                         
GATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAACCCCACAAAGCCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATG  >  NZ_CP009273/2370687‑2370844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑