| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,357 | 0 | A | T | 70.6% | ‑3.4 / 12.6 | 17 | intergenic (+47/‑56) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base A (0/5); new base T (12/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACT > NZ_CP009273/2380271‑2380446 | aGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACattttattt < 1:1567814/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:282367/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:1393715/90‑1 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 2:1286658/90‑1 (MQ=255) aCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGCACATTTTTTTTTTCCTCCTCttttttt > 2:116597/1‑89 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGtttttaa > 2:282367/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAAGACAATTTTTTTTTTCTCATCtttttttat > 1:891763/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACAAGCACATTTTTTTTTTCCTCCTCTGtttttaa > 2:1308140/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCATCtttttttaa > 1:276552/1‑87 (MQ=255) tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGAAAATTTTTTTTTTTCTCCTCTGTTTTTAGaa > 1:119222/1‑89 (MQ=255) ataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGa < 2:1565515/90‑1 (MQ=255) acaacaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTTTCTCCTCTGTTTTTAAATGCGaaa > 1:1227963/1‑90 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAAAAAAGCACAATTTTTTTTTTCCCCTCTTTTTTTAGAAGAGaaaaaa > 1:849369/1‑87 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACATGCACATTTTTTTTTTCCTCCTCTTTTTTTAGATGGGAAAAATGaaaa > 1:8663/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAACATGAAATTTaaa > 2:142264/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCATCTGTTTTTAGATGCGAAAAATGAAATTCaaa > 2:1045201/1‑89 (MQ=255) aTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 2:707936/90‑1 (MQ=255) tttCTCGAGGCGGGTCCCGAAAAAAAGAAAAACACAATTTTTTTTTTCCCCTCTTTTTTTAAATGAGAAAAAAGAAAATAAAAAGAGGaa > 1:1567594/1‑90 (MQ=255) ttttattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt < 2:1160524/90‑1 (MQ=255) tattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGAc < 2:1567594/90‑1 (MQ=255) attttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt < 2:807626/90‑1 (MQ=255) | AGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACT > NZ_CP009273/2380271‑2380446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |