| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,408,014 | 0 | A | T | 54.5% | 6.1 / 13.2 | 11 | N57K (AAT→AAA) | ralR | type I toxin‑antitoxin system endodeoxyribonuclease toxin RalR |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base T (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGC > NZ_CP009273/1407931‑1408103 | ttGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTAATGAAAACCCCCCTtttttttttta > 2:192256/1‑87 (MQ=255) atCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGtt < 1:85787/90‑1 (MQ=255) gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTTTAATAAAACCCCCCTTATTTTTTTCCCTGGGTTGTTTTTTTCCTCtttttag > 1:634693/1‑86 (MQ=255) gTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAAAACCTCCCTTTTTTTTTTCCGTGGGTTGTTTTTTTCCTCttttgtg > 1:225485/1‑86 (MQ=255) gCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTACATGAAAACCTCCCTTTTTTTTTTCCGTGGTTTGTTTTTTTCATTTTTTGGGTg > 1:371692/1‑90 (MQ=255) gCCACCTTCCTTTTCAATAGTGGCGGTAATTTTTTAAATGAAAACCTCCCTTTTTTTTTTCCGTGGGTTGTTTTTTTCATCTTTTGAGTg > 1:222929/1‑90 (MQ=255) ccttTTCAATAGTGGCGGTAATTTTTTACATTAAAACCCCCATTTTTTTTTTCAGTGGTTTGTTTTTTTCCTCTTTTTAGTGGTtttttt > 1:5539/1‑90 (MQ=255) ttttCAATAGTGGCGGTAATTTTTTAAATGAAAACCCCCCTTATTTTTTTCATTGGGTTGTTTTTTTCCTCTTTTGAGTGCTTCtttttt > 2:137890/1‑89 (MQ=255) tAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCg < 1:514558/90‑1 (MQ=255) tataCATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCgg < 1:23316/90‑1 (MQ=255) aaCACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCa < 2:29636/90‑1 (MQ=255) tCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGtt > 2:298561/1‑90 (MQ=255) attattattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTaca > 1:682197/1‑90 (MQ=255) tattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCg > 2:153224/1‑90 (MQ=255) tattTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCg > 2:8533/1‑90 (MQ=255) attTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGc < 1:298561/90‑1 (MQ=255) | TTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGGTAATTTTATACATGAACACCTCCATTATTATTTCCAGTGGTTCGTTTATTCCATCTTTCGAGTGCTTCTTTTTCACTTCCACCATAACCGGTTCGGGATTCGCATCCGTTACACTTCGC > NZ_CP009273/1407931‑1408103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |