| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,379 | 0 | C | G | 54.2% | ‑2.3 / 10.3 | 24 | G72G (GGC→GGG) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base C (2/9); new base G (6/7); total (8/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.07e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC > NZ_CP009273/3738291‑3738465 | tGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGGAAACATGAATgcgggcg > 2:242091/1‑90 (MQ=255) aaCGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGGAAACATGAATGCGGGGGGCGTggc > 2:617245/1‑88 (MQ=255) cGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCaa < 2:221163/90‑1 (MQ=255) cTGGTGAACGGAGCCGATAGCTTCTCACTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGGAATCATGAATGCGGGGGGCTTGTCaaaa > 1:306843/1‑90 (MQ=255) gTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGc < 1:631837/90‑1 (MQ=255) gAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAAAAATGAATGGGGGGGGCTTGTCAAAACGCAt > 1:233350/1‑90 (MQ=255) ccGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGAcc < 1:242091/90‑1 (MQ=255) aaTAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTTTCAAAAAGCATTTTTGACCtt > 1:526812/2‑89 (MQ=255) cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGGGGCTTGTCAAAACGCATTGTTGACCTGCCGAt > 1:82790/1‑90 (MQ=255) cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAAAAATGAATGCGGGGGGCCTTTCCAAACGCATTGTTGGCCCGCCCAt > 2:734058/1‑90 (MQ=255) ttCTCACTGCTGGCGATTCCGTTTTTTGTGCGGGGGGGTGAAATCATGAATGCGGGGGGTTTGTCAAAACGCATTGTTGCCCTGCCGATg < 2:306843/90‑1 (MQ=255) tctcCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAAACATGAATGCGGGGGGCTTGTCAAAACGCATTGTTGACCTGCCGATGa > 1:636297/1‑90 (MQ=255) cccTGCTGGCGTTTCCTTTCTTTGTGCTGGGGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAAc < 2:297197/90‑1 (MQ=255) ccTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACt < 1:601701/90‑1 (MQ=255) gctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGGTGAAACtggt > 1:289045/1‑90 (MQ=255) ctgGCGTTTCCGTTTTTTGTGCTGGGGGGTGAAATCATGAAGGCGGGCGGTTTGTCAAAACGCATTGTTGACCTGCCGATGAAACtggtg < 2:75485/90‑1 (MQ=255) ctgGCGATTCCTTTTTTTGTGCGGGGGGGGAAAATCATGAAGGGGGGGGGTTTGTAAAAACGCATTGTTGCCCTGCCGATGAAACtggtg < 2:546060/90‑1 (MQ=255) tgGCGTTTCCGTTTTTTGTGCTGGGGGGTGAAATCATGAATGCGGGCGGCTTGTAAAAACGCATTGTTGACCTGCCGATGAAACtggtgg < 2:263115/90‑1 (MQ=255) gATTCCTTTTTTTGTGCTGGGGGGTAAAATCATGAATGCGGGGGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCa < 2:233350/90‑1 (MQ=255) ttCCGTTCTTTGTGCTGGCGGGTGAAATCATGAAGGGGGGGGGTTTGTCAAACCGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATa < 2:540667/90‑1 (MQ=255) tgCTGGGGGGTGAAATCATGAAGGGGGGGGGTTTGTAAAAACGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 2:803298/90‑1 (MQ=255) tgCTGGGGGGTGAAAACAAAAATGCGGGGGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:780724/90‑1 (MQ=255) tgCTGGCGGGTGAAATCATGAATGCGGGCGGTTTGTCAAAACCCTTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:668493/90‑1 (MQ=255) tgCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:314508/90‑1 (MQ=255) aTGAAGGGGGGGGGTTTGTAAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTg < 2:800192/90‑1 (MQ=255) gggcggCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGc > 2:31615/1‑90 (MQ=255) | TGGCACAAACGCTGGTGAACGGAGCCGATAGCTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGC > NZ_CP009273/3738291‑3738465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |