| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,774,215 | 0 | T | G | 61.8% | 18.9 / 29.9 | 34 | G343G (GGT→GGG) | lldD | quinone‑dependent L‑lactate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base G (21/0); total (26/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.09e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.54e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAA > NZ_CP009273/3774130‑3774293 | tCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAg > 2:73574/1‑90 (MQ=255) cGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGc > 2:289148/1‑90 (MQ=255) cGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGc > 2:267929/1‑90 (MQ=255) gTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAAcc > 1:106222/1‑90 (MQ=255) gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAAttt > 2:325054/1‑88 (MQ=255) gcTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCt > 2:636540/1‑90 (MQ=255) cTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGAGCTAACCTGCTAAATCTg > 2:290363/1‑90 (MQ=255) gTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATTTGATCg > 1:447151/1‑90 (MQ=255) ccGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGaaa < 1:796432/90‑1 (MQ=255) cagccGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAag < 1:766097/87‑1 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAagaggt > 2:619640/1‑88 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAAACTGATCGAAAAAGAGAt > 2:147550/1‑90 (MQ=255) cGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGGGGCGAACCTGCTAAATCTGGTCGGAAAaaaggt > 2:594712/1‑88 (MQ=255) tACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGa > 1:43559/1‑90 (MQ=255) tgctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAg < 1:73574/90‑1 (MQ=255) gctgGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAgg > 1:213894/1‑89 (MQ=255) gggTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAggggg > 2:544899/1‑89 (MQ=255) gggTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAggggg > 2:373861/1‑89 (MQ=255) gggTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAAACTGATCGAAAAAAAGAAGAAAGtctg > 2:766097/1‑87 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTGGCTAACCTGCTAAATCTGTTTGAAAAAGAGATGAAAGAggggg > 1:751930/1‑87 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGa > 2:96182/1‑90 (MQ=255) gTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGTGGCGa > 2:180334/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGa > 2:682639/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGGTGAAAGTGGCGATGa > 2:405319/1‑90 (MQ=255) gTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGaggg > 1:552824/1‑87 (MQ=255) gTGCTTTCTTGTATGCGCTGGAAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGa < 2:751930/90‑1 (MQ=255) ttGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACt < 1:417963/90‑1 (MQ=255) gTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGaaa < 2:247591/59‑1 (MQ=255) gTATGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGaaa > 1:247591/1‑59 (MQ=255) aTGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTTATCGAAAAAGGGATGAAAGGGGGGATGACGCTGACTgggg > 2:679228/1‑88 (MQ=255) aTGCGCTGGCAACAGCGGGCCAGGCGGGGGTAGCTAACCTGCTAAATCTGATCGAAAAAAAGATGAAAGTGGGGGTGACGCTGGCTGgcg > 2:684878/1‑90 (MQ=255) cAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGa < 1:679228/90‑1 (MQ=255) aCAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATc < 1:704334/90‑1 (MQ=255) ggCCAGGCGGGTGTAGCTAACCTGCTAAATCTTATCGAAAAAGAGAGGAAAGGGGGGGGGGCGCTTGCTGGGGCGAAATCGATCAGCGaa > 2:630132/1‑90 (MQ=255) | TCGTGCGTATGATTGCGCTCGGTGCCGACACCGTACTGCTGGGTCGTGCTTTCTTGTATGCGCTGGCAACAGCGGGCCAGGCGGGTGTAGCTAACCTGCTAAATCTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAA > NZ_CP009273/3774130‑3774293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |