| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,500,285 | 0 | A | C | 56.0% | 24.2 / 32.0 | 25 | V18G (GTA→GGA) | ydcO | BenE family transporter YdcO |
| Reads supporting (aligned to +/- strand): ref base A (9/2); new base C (0/14); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACC > NZ_CP009273/1500197‑1500369 | cATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATAc < 1:1175282/90‑1 (MQ=255) tCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCg < 1:1312191/90‑1 (MQ=255) agagTTTTGGGCTGTGGGGGCCCCGGCGAAAATCGCTGCTGGCAAGATTATTGCCGCTGAATTGGCGAACCAAATAAATCCCGCCAGaac < 1:949632/90‑2 (MQ=255) ggtggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgg > 1:1418432/1‑89 (MQ=255) ggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtggg > 2:3599/1‑90 (MQ=255) ggCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCgtggg > 1:1303338/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:1095640/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:837009/1‑90 (MQ=255) cGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTggag > 1:181295/1‑90 (MQ=255) ggCAACAACCGCTGCTGGCCATTTTTTGGCCGCGGACGGGGGGTAGAAAATAAACCCCGCAAAAACCCCCGCCAGTAGCGTGGGTggagg < 1:703359/90‑1 (MQ=255) ctTGCCAGATTATTGCCGCTGAACGGGGGTACCCAATTAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 2:1384280/90‑1 (MQ=255) ctTCCCAGATTATTGCCGCTGAACGGGCGTACCAAATAAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGAc < 2:610592/90‑1 (MQ=255) tcccAGATTATCGCCGCTGACCGGGCGTCACAAATAAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGc < 2:1392151/88‑1 (MQ=255) tcccAAATTATTGCCGCTGAACGGGGGTAGCCAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGc < 2:537899/88‑1 (MQ=255) gCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCa > 1:1052227/1‑90 (MQ=255) ccAGATTATTGCCGCTGAACGGGCGTAGCCAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCAt < 2:486442/90‑1 (MQ=255) ttGCCGCAGAACGGGGGAAGCAAATAAACCCCCCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCt < 2:1042858/90‑1 (MQ=255) cccGCTGACCGGGCGTACCCAATTAATCCCGCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCg < 2:8494/89‑1 (MQ=255) gCTGAACGGGAGTACCAAATTAATCCCGCAAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGc < 2:1095640/90‑1 (MQ=255) cgggCGTACCCAATAAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata < 2:1418432/88‑1 (MQ=255) cgggCGTACCAAATAAACCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTtata < 2:251902/88‑1 (MQ=255) ggCGTAGCAAATTAATCCCCCCAAAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGc < 1:3599/90‑1 (MQ=255) aGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACa > 1:356416/1‑90 (MQ=255) aTTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAc > 1:586056/1‑90 (MQ=255) ttAATCCCGCCAGAACCCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAGCAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCAcc < 2:512697/90‑1 (MQ=255) | CATCCAGCCAGAGATTTGTGCAGTGGTGGCTCCGGCGACAATCGCTGCTTGCCAGATTATTGCCGCTGAACTGGCGTAGCCAATTAATACCGCCAGAAACCCCGCCAGTAGCGTGGGTGGAGGAATAGAAAACAGACGCATAGTCACCTCGTGCGTTATAGCGTACAGGCACC > NZ_CP009273/1500197‑1500369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |