| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,029,116 | 0 | A | C | 54.2% | 21.6 / 32.8 | 24 | G175G (GGT→GGG) | prfB | peptide chain release factor 2 |
| Reads supporting (aligned to +/- strand): ref base A (11/0); new base C (0/13); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGC > NZ_CP009273/3029035‑3029200 | cACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCAc > 2:648108/1‑90 (MQ=255)cACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCAc > 2:1286959/1‑90 (MQ=255) aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCa > 2:6522/1‑90 (MQ=255) aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCa > 2:1040141/1‑90 (MQ=255) aGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCa > 1:787498/1‑90 (MQ=255) ggTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCtt > 1:1398402/1‑90 (MQ=255) aaCGCGGTTTTCTGTCCGCACCCGGCCGAAAGCGTAACCGCCGGATTTTTTGATCGTCACGGTTTT‑AATCCCCGCCACTTCACCTTCCGa < 2:309113/90‑1 (MQ=255) aaCGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGa > 2:358485/1‑90 (MQ=255) cGCAGCCAGCCGAAAGCGTAACCGCGGGATTTTTTGATCGTC‑CGGTTTTAAATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCa < 2:1329774/90‑1 (MQ=255) cGTAAGCTTAATCGCGGGATTTTTTGATCGTCAGTTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAAc < 2:222098/90‑1 (MQ=255) tAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCa > 2:693062/1‑90 (MQ=255) gcttAACCGCGGGAGTTTTTGTTCGTCAGGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 1:452171/87‑1 (MQ=255) gCGTAACCGCGGGATTTTTTGTTCGTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCAcgc < 1:487660/90‑1 (MQ=255) aaTCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGAtt > 1:346854/1‑90 (MQ=255) gggAGTTTTTGATCGTCACGGTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc < 2:252983/89‑1 (MQ=255) cGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGccc > 1:825660/1‑90 (MQ=255) tttttGATCGTCATGATTTT‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAgcgc < 2:1440206/89‑1 (MQ=255) tttGTTCGCCACGTATTA‑AACCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAg < 2:1274782/90‑1 (MQ=255) tgtccGTCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat < 1:1343132/86‑1 (MQ=255) tGATCGCCACGGTTTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGat < 2:346854/90‑1 (MQ=255) gTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacata > 1:291468/1‑90 (MQ=255) tCACGTTTTT‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGatacatac < 2:593847/90‑1 (MQ=255) aCGAATTA‑AATCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGc < 2:74860/90‑1 (MQ=255) taaaTCCCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGc < 2:194171/88‑1 (MQ=255) | CACCAGGCGGTGAACGCCGGTTTCTGTACGCAGCCAGCCGTAAGCGTAATCGCCGGAGATTTTGATCGTCACGGATTT‑AATACCCGCCACTTCACCTTCCGACTCTTCGATGATTTCAGTTTTGAAACCACGCGATTCTGCCCAGCGCAGATACATACGCTCAAGC > NZ_CP009273/3029035‑3029200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |