Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,594,1670CT59.0% 12.6 / 100.6 84S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
Reads supporting (aligned to +/- strand):  ref base C (5/29);  new base T (49/0);  total (54/30)
Fisher's exact test for biased strand distribution p-value = 1.44e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.67e-14
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGG  >  NZ_CP009273/4594078‑4594256
                                                                                         |                                                                                         
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  1:618713/90‑1 (MQ=255)
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  1:1241298/90‑1 (MQ=255)
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  1:68232/90‑1 (MQ=255)
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  2:414804/90‑1 (MQ=255)
cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  1:596468/90‑1 (MQ=255)
  cTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                         <  1:1394081/90‑1 (MQ=255)
    tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                           <  2:740772/86‑1 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATgaga                                                                                 >  2:1251260/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATgaga                                                                                 >  1:612949/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGGCTTTTTTTTTTTTTTGCCAttgga                                                                                 >  1:1251641/1‑86 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATgaga                                                                                 >  2:448534/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCATgaga                                                                                 >  1:1145314/1‑90 (MQ=255)
          cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACCTTTTTTTTTTTTTTTCCCTgaga                                                                                 >  2:673952/1‑90 (MQ=255)
              aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCGTTTTTTTTTTTTTTTCCTTGTGAAggg                                                                             >  1:951944/1‑88 (MQ=255)
                 ccTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                                          <  2:846369/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTTAGAAATGGgcgc                                                                        >  1:1598760/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAgcgc                                                                        >  1:624293/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAgcgc                                                                        >  2:972129/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAgcgc                                                                        >  2:374372/1‑90 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcgc                                                                        <  1:1244114/90‑1 (MQ=255)
                   tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTGCCCTGAGAAGGGGgcgc                                                                        >  1:1450802/1‑90 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCa                                                                       >  2:18267/1‑90 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGAAGTGAgggcc                                                                       >  1:753012/1‑89 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGGCATTAGGAGTGAgagcc                                                                       >  2:801171/1‑89 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCa                                                                       >  1:1526392/1‑90 (MQ=255)
                    ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCa                                                                       >  1:76217/1‑90 (MQ=255)
                     taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                      <  1:256462/90‑1 (MQ=255)
                     taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                      <  1:1543748/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  2:1287484/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  2:1567800/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  2:612949/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  2:972397/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagaga                                                                   <  1:1185382/90‑1 (MQ=255)
                        cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCTTTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCagggg                                                                   >  1:744604/1‑87 (MQ=255)
                         cgTCAGGAGTCATTTAAGGATGGACCGCCGCGGATGTGGTTTTCGGACGGCATTTTTGCCTTGTATGGCATGAGGAGTGACCGCcgagag                                                                  <  1:1166846/89‑1 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCagggag                                                                  >  2:751721/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCagagag                                                                  >  1:5114/1‑90 (MQ=255)
                         aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCagagag                                                                  >  2:246194/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                                 >  1:1224496/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTGCATGGGAAGAGAGCGCAGGGAgg                                                                 >  1:577460/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAgggg                                                                 >  1:212001/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGGGGGCAGAGAgg                                                                 >  2:256462/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAgg                                                                 >  2:1131318/1‑90 (MQ=255)
                          gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGGGAAGTGGGGGCAGAGAgg                                                                 >  2:1281831/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCACTAGAAGTGAGCGGAGAGAggg                                                                >  1:372116/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAggg                                                                >  1:1201216/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggg                                                                >  1:1008529/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGAGAGCGCCGAGAggg                                                                >  2:1069529/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGgggg                                                                >  1:159845/1‑90 (MQ=255)
                           tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGAAGAGAGCGGAGAGAggg                                                                >  1:14712/1‑90 (MQ=255)
                            cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGa                                                               >  2:720844/1‑90 (MQ=255)
                            cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                               <  2:228938/90‑1 (MQ=255)
                            cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                               <  1:448534/90‑1 (MQ=255)
                              ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGGAGGGGGGGGtt                                                             >  1:1031766/1‑90 (MQ=255)
                              ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGTGGGGGCAGAGGGGGGtt                                                             >  2:1543748/1‑90 (MQ=255)
                              ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAgggggt                                                             >  1:1320062/1‑87 (MQ=255)
                                 gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                          <  1:673952/90‑1 (MQ=255)
                                  tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                         <  1:298920/90‑1 (MQ=255)
                                   cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGTTATCtt                                                        >  2:951175/1‑90 (MQ=255)
                                    aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGGTATCTTg                                                       >  2:489252/1‑90 (MQ=255)
                                     tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                      <  1:18267/90‑1 (MQ=255)
                                     tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                      <  1:533862/90‑1 (MQ=255)
                                        cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctg                                                   <  2:211960/90‑1 (MQ=255)
                                             tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTCCGAAGGGAGGGCCGGGAGGGATTTTTTTGCccgactga                                              >  1:744056/1‑90 (MQ=255)
                                              gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGGAGTGAGGGGGGGGGGGGGTTATCTTTCctgtctgta                                             >  1:576429/1‑88 (MQ=255)
                                              gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGGGGGGGGTTATTTTTCCTTTCTGaa                                             >  1:112901/1‑90 (MQ=255)
                                               ccgtGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATTAGAAGGGGGGGCAGGGGGGGGTTATCTTTCCTGACTGAAc                                            >  2:131118/4‑90 (MQ=255)
                                               ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                                            >  2:94139/1‑90 (MQ=255)
                                                cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACt                                           <  1:172989/90‑1 (MQ=255)
                                                   gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCGTTAGAAGTGGGCGCCGGGGGGGGGTATTTTGTCTGACTTAACTGGc                                        >  1:1572675/1‑90 (MQ=255)
                                                      gcgGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCGGAGGGGGGTTTTTTTGTCTGACTGAACTGGGGAt                                     >  1:325396/1‑90 (MQ=255)
                                                        ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGGAGGGAGCGCAGGGGGGGGTTTTTTTGCCTGACTGAACTGGCGATTa                                   >  1:836477/1‑90 (MQ=255)
                                                        ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATTTTGCCTGACTGAAATGGCGATTa                                   >  2:1500559/1‑90 (MQ=255)
                                                        ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTa                                   >  2:515527/1‑90 (MQ=255)
                                                             tcGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGAGAGGGGTTATTTTTCCTGTCTGAACTGGCGGTTTAGttt                              >  1:122920/1‑90 (MQ=255)
                                                             tcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGGTTTAGttt                              >  2:1243465/1‑90 (MQ=255)
                                                             tcGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGAGAGGGGGTTTTTTTCCTGACTGAACTGGGGATTAAGttt                              >  1:1029327/1‑90 (MQ=255)
                                                              cGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGGGCGCAGAGGGGGGTTATTTTTTCTTACTGAACTGGCGGTTAAGtttt                             >  2:534225/1‑89 (MQ=255)
                                                                      gcggTCATTTTTTCTTTTTCTGCCATGAGGAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                     <  2:1139491/90‑1 (MQ=255)
                                                                       cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                    <  1:640115/90‑1 (MQ=255)
                                                                       cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                    <  2:1008529/90‑1 (MQ=255)
                                                                        ggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg                   <  1:1273147/90‑1 (MQ=255)
                                                                        ggTCATTTTTTCTTTTTCTGCCATGAGAAGGGGGGGCAGAGAGGGATTATCTTGCCTGTCTGAACAGGGGGTTAAGTTTCCGCGTACCCg                   >  2:664651/1‑90 (MQ=255)
                                                                         gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                  <  1:131118/90‑1 (MQ=255)
                                                                         gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                  <  1:94139/90‑1 (MQ=255)
                                                                          tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGc                 <  2:753012/90‑1 (MQ=255)
                                                                            aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt               <  2:569598/90‑1 (MQ=255)
                                                                            aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGt               <  2:1593763/90‑1 (MQ=255)
                                                                             ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt              <  1:657937/90‑1 (MQ=255)
                                                                             ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGtt              <  1:720844/90‑1 (MQ=255)
                                                                              tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt             <  1:1500559/90‑1 (MQ=255)
                                                                               ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa            <  2:577460/90‑1 (MQ=255)
                                                                                     ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt      >  2:1596044/1‑90 (MQ=255)
                                                                                     ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt      >  2:1350461/1‑90 (MQ=255)
                                                                                     ttttctGCCATGAGAAGCGAGCGCAGAGAGGGATTATCTTGCCTGACTGCACTGGCGATTAAGTTTTCTGGTACCCGGCGTTTAGTTAtt      >  1:375597/1‑90 (MQ=255)
                                                                                       ttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc    <  1:1596044/90‑1 (MQ=255)
                                                                                       ttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc    <  1:1346204/90‑1 (MQ=255)
                                                                                       ttctGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGGTTCCGCGTACCCGGCGTTTAGTTATTGc    >  1:400251/1‑90 (MQ=255)
                                                                                         ctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCgg  <  1:15016/90‑1 (MQ=255)
                                                                                         |                                                                                         
CCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGG  >  NZ_CP009273/4594078‑4594256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑