| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 899,438 | 0 | A | C | 51.4% | 31.9 / 49.8 | 35 | G162G (GGT→GGG) | ybjP | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (15/2); new base C (0/18); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.19e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGCATTCCTCACGAAATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTG > NZ_CP009273/899352‑899527 | cGGCATTCCTCACGAAATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTAccc > 2:1077174/1‑90 (MQ=255) ttCCTCACGAAATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGt > 2:330943/1‑90 (MQ=255) gAAATGCCGGACATTTTAGGGGTTTTTTTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGGGTACCCGCCGTGGCATGaa < 2:1551290/90‑1 (MQ=255) tGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCt > 2:15109/1‑90 (MQ=255) cgaaCATTTTAGGGGGTTTATTGGTTGATAAAGGCGTTAGCGTTTCTCGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTAcc < 2:60609/87‑1 (MQ=255) aaattttAGGGGTTTTATTGGTTGAACAGGGCGTTAGCGTTCCTCGAGGGACGGACGGGGCGTCCCCGCCGTGGCATGAACGCTACCGcc < 1:723637/86‑1 (MQ=255) attttAGGGGTTTTTTTGGTTAACAAGGGGGTTAGCTTTTCCCGAGGGACTGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTa < 1:1269823/88‑1 (MQ=255) tgggggtTTTTTTGGTTGATAAGGGCTTTAGGGGTTCTAGAGGGACGGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTa < 2:61124/87‑1 (MQ=255) ggggtTTTATTGGTTGATCAAGGCTTTAGCTTTTCCCGATGGCCTGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAAc < 2:1311904/89‑1 (MQ=255) ggTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTA‑CCGCCGTGGCATGAACGCTACCGCCTAAGTAACGcac > 1:1253976/1‑90 (MQ=255) tttATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGcaca > 2:779470/1‑90 (MQ=255) tttATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGcaca > 2:1323884/1‑90 (MQ=255) tttttGGTTGATCAGGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACAt < 2:544582/87‑1 (MQ=255) tttttGGTTGAACAGGGCGTTAGCGTTTCCGGAGGGACGGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACAt < 1:74556/87‑1 (MQ=255) tATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACAtc > 1:1003683/1‑90 (MQ=255) gTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGa > 1:927007/1‑90 (MQ=255) ttaaTCAGGGCGTGAGCATTTCTCGAGGGCCTGAGGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:1047676/87‑1 (MQ=255) ttGATCAGGGCGTTAGCGATTCCCGATGGACTGACGGGGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGAt < 2:1107883/90‑1 (MQ=255) cagggCGTTAGCGACTCTGGAGGGACGGCGGGAGCGCCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGAccc < 1:467509/87‑1 (MQ=255) aaaGGCTTTAGCGATTCTCGAGGGACTGACGGAGCTTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGAccc < 2:1545198/89‑1 (MQ=255) aGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCaa > 1:1107883/1‑90 (MQ=255) ggCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAac > 2:871312/1‑90 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 2:302396/1‑90 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 1:1036934/1‑90 (MQ=255) tAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTgg > 1:289007/1‑90 (MQ=255) gctaTTCTCGATGAACTAAGGGGCGGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGcc < 1:116377/87‑1 (MQ=255) ctcGATGGACTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTg < 2:481554/90‑1 (MQ=255) cGAGGGACTGAGGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGaa < 2:512351/90‑1 (MQ=255) cggACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTaa < 2:675939/88‑1 (MQ=255) cTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTaa < 2:289007/90‑1 (MQ=255) cTGACGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTaa < 2:903131/90‑1 (MQ=255) gACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAaca > 2:151784/1‑90 (MQ=255) aCGGAGCGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTg < 1:1465847/78‑1 (MQ=255) aCGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTg > 2:1465847/1‑78 (MQ=255) cGTCCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATc < 2:1003683/90‑1 (MQ=255) aCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTGATCCTg > 1:615746/1‑90 (MQ=255) aCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTg > 2:1204836/1‑90 (MQ=255) aCCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTg > 2:1103074/1‑90 (MQ=255) | CGGCATTCCTCACGAAATGCCGGACAATTTACGGGGTTTATTGGTTGATCAAGGCGTTAGCGATTCTCGATGGACTGACGGAGCGTACCCGCCGTGGCATGAACGCTACCGCCTAAGTAACGCACATCGTCGATGACCCAACACTGGCCTTCCTGAATCATTAACACTTCATCCTG > NZ_CP009273/899352‑899527 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |